Anti-oxidized LDL antibodies in patients with abdominal aorta aneurysm and arteriosclerosis obliterans

Abstract number: P0687

Doskocz R. E., Knapik-Kordeck M., Adamiec R.

Medical University, Wroclaw, Poland

Introduction  

Pathogenesis of aortic aneurysm is mainly atherogenetic with inflammation features [1]. Formation of atherosclerotic plaque is based on inflammation also. The process starts from LDL oxidation. LDL promotes growth of arterial smooth muscle cells (SMCs) and oxidized LDL (oxLDL) promote apoptosis in vascular (SMCs) [1]. It has been shown that LDL oxidation stimulates defensive system to produce Immunoglobulin IgG anti-oxLDL, isolated from atherosclerotic plaque. Specific reaction between atherosclerotic plaque and IgG has been indicated. Appearance of anti-oxLDL was described in healthy and in several diseases, especially in atheromatosis [2]. In present work we estimated anti-oxLDL antibodies (oLAB) in patients operated on because of arteriosclerosis obliterans, abdominal aorta aneurysmata and in healthy control group.

Material and methods  

We qualified: 29 patients aged 67.2 (+/7.29) with abdominal aorta aneurysmata proved by USG and CT scan, 39 patients aged 61.05 (±8.9) qualified to prosthesis Y or simple implantation, in stage III or IV ischemia according to Fontaine 15 healthy persons aged 37.4 (± 10.6). Blood was collected from ulnar vein. It was stored in -38 °C temperature. Anti-oxidized LDL antibodies were estimated by immunoenzymatic micromethod ELISA using commercial Kit ‘oLAB’ manufactured by Biomedica.

Summary  

Mean level of oLAB in patients with aneurysmata was 376.45 (±34) mU mL^-1; in patients with arteriosclerosis obliterans – 289.67 (±28) mU mL^-1, and in control group – 561 (±43) mU mL^-1. Statistical significant decrease of oLAB level was shown in patients with atheromatosis and aneurysmata comparing to control group (P = 005) and (P = 0.01) adequately.

Conclusion  

Above results may show the protective role of estimated antibodies against atheromatosis and aneurysmata in younger population, because in these diseases they are bound with oxidized LDL and don't protect vascular wall against the atheromatic progress as much as in earlier stages. Age dependent decrease of o-LAB in healthy people also was described in literature before.

1  Okura, Y, et al.Oxidized low-density Lipoprotein is associated with apoptosis of vascular smooth muscle cells in human atherosclerotic plaques. Circ 2000; 102: 2680.

2  Cherubini, , et al.Autoantibodies against oxidized low density lipoproteins in older stroke patients. J Am Geriatr Soc 1997; 45: 125.