2-Arachidonoylglycerol (2-AG) endocannabinoids, induces platelets conjugation with neutrophils (Platelet Satellitism) in vitro

Abstract number: CD061

Kawahara^* K., Abeyama† K., Yamaji† K., Maruyama† I.

†Japan ^*Kagoshima University, Japan;

Conjugates between platelets and neutrophils, so-called ‘Platelet Satellitism’, are believed to result in a mutual modulation of cellular responses and to play an important role in the early phase of inflammatory processes. However, the molecular and cellular mechanism in the formation of Platelet Satellitism has remained to be elucidated. On the other hand, Recent studies have implicated that 2-arachidonoylglycerol (2-AG) including esters of unsaturated fatty acid is a novel class of lipid mediator in the early inflammatory process. 2-AG is generated promptly from the lipid of cytoplasm membrane without gene transcription. In this study, we examined the relationship between Platelet Satellitism and 2-AG, both are generated at the early phase of the inflammatory process. 2-AG strongly induced Platelet Satellitism in vitro. Platelet Satellitism induced by 2-AG is mediated between GPIIb/IIIa (CD41) on platelet and Mac-1 (CD11b) on neutrophils. Furthermore, SR144528 antagonist of 2-AG was inhibited Platelet Satellitism induced by 2-AG in this study. We conclude that the stimuli-induced 2-AG generation could strongly influence Platelet Satellitism, increasing and enhancing their functional capacity during an early phase of inflammatory process.