The survey of changes in complete blood count (CBC) and RBC indices at different temperatures up to 48 h
Abstract number: V1-062P
Mahmoodi1 M., Asadikaram1 G., Khaksari2 M., Seyedi1 N., Rahnema3 A., Hajizadeh1 M., Mirzae1 M., Sayadi4 A.
1Department of Biochemistry and Biophysics, Faculty of Medicine, Rafsanjan Medical University, Rafsanjan, Iran 2Department of Physiology, Faculty of Medicine, Rafsanjan Medical University, Kerman, Iran 3Department of Pathology, Faculty of Medicine, Rafsanjan Medical University, Rafsanjan, Iran 4Department of Psychology, Faculty of Medicine, Rafsanjan Medical University, Rafsanjan, Iran. E-mail: mahmoodies@yahoo.com
Background and objectives:
CBC is one of the most common and conventional blood test that physicians usually request. The result of this test is effective in physician's decision to diagnosis and treatment. However the results of this test are affected by different factors such as, the temperature and duration of incubation, duration between sampling and doing the test, patient situation, therefore the aim of this survey was to evaluate the effect of temperature and time of incubation on CBC, RBC indices and white blood cells (WBC) differential count in a cross-sectional study.
Materials and methods:
Blood samples were taken from 30 healthy medical students of Rafsanjan University (15 males and 15 females). The samples divided into three parts; CBC were done on the samples up to 48 h incubation at temperature of 25, 30, and 37 °C at the time of sampling, and after 2, 8, 24 and 48 h. Data were statistically analyzed and the following results were obtained.
Results:
RBC count, hematocrit, MCH, percent of monocytes and eosinophils were constant in different temperatures, WBC count, MCHC, hemoglobin, platelets count, the percent of lymphocytes and neutrophils were constant up to 24 h and then tend to increase with increasing temperature except lymphocytes percent that tend to decrease. MCV decreased with increasing temperature up to 8 h and then increased. WBC, hematocrit, MCV, platelets count, and neutrophils’ percent tend to increase by the time of incubation, but RBC count, MCHC, lymphocytes’ percent decreased. Hemoglobin, MCH, and the percent of monocytes and eosinophils were constant.
Conclusion:
The finding of this survey showed that some of CBC parameters can be changed with the incubation, therefore it is better to do the CBC test after blood taking as soon as possible.
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