Relationships between ascorbic acid, hydroxyproline and collagen, of wound healing in diabetic rats
Abstract number: H2-014P
Khaksari Haddad1 M., Rezaizadeh2 A. R., Mardani3 M., Asadi Karam4 G. R., Mahmoodi4 M.
1Department of Physiology, Kerman Medical University, Kerman, Iran 2Department of Anatomy, Rafsanjan University of Medical Sciences, Rafsanjan, Iran 3Department of Anatomy, Isfahan University of Medical Sciences, Isfahan, Iran 4Department of Biochemistry, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. E-mail: khaksar38@yahoo.co.uk
It was reported that increased oxidative stress in diabetes mellitus associated with decrease in collagen synthesis. In this present study, the role of ascorbic acid on hydroxyproline and collagen in diabetic rats is reported. This study was performed on 160 male rats. Diabetes was induced by injection of streptozotocin [STZ, S.C , 55 mg/kg].Animals were divided into four groups, and then rats were remained diabetic for 8 weeks. Control group (I), received the vitamin c deficient diet. Prophylaxia group (II), received 250 mg/lit vitamin c, one month before induction of diabetes. Treatment group (III), received vitamin c after induction of diabetes. Combination group (IV), received the vitamin c one month before diabetic induction, until wound healing. Full thickness incision induced on back skin of rat, 8 weeks after diabetic induction. Content of collagen and biochemical measurement of hydroxyproline at days 1, 3, 7, 11.15, and 20 after operation were measured. The results showed, the mean of collagen content and hydroxyproline in group I was more than all examined groups, at days of 11, 15 and 20, in addition, in group III this indication was less than group IV during all days. This data suggest that, vitamin c supplementation in chronic diabetic rats impaired wound healing, and decreased collagen and hydroxyproline content, while used as prophylaxis or combination.
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