Serotypes distribution and susceptibility to antibiotics of Streptoccus pneumoniae isolated in blood cultures in Madrid, Spain

Abstract number: R2389

Moreno M., De Las Cuevas C., Agudo S., Martínez M., López-Brea M., Sanz J.

Objective: This study was to determine the serotypes distribution of Streptoccus pneumoniae isolated from adult patients with bacteremia for three years.

Methods: 77 samples were isolated from blood cultures in adult patients. Samples were received at the Department of Microbiology (Hospital Universitario de la Princesa, Madrid, Spain) from January 2007 to November 2009. Seventy seven patients were studied of whom 41 males and 36 females and were a age range between 25–98. The microorganism was identified by being susceptibility to optochin and soluble to bilis. Susceptibility to penicillin, cefotaxime, erithromicin and levofloxacin was determined by E-test. Clinical resistance was based on CLSI (M100S13) 2008 breakpoints. Serotype was determined using rapid latex agglutination (Pneumotest Latex, Statens Serum Institut, Denmark) and specific factors of serotyping were performed through Quellung reaction.

Results: Seventy nine strains (89.6%) were included in the 23 valent vaccine (VP23V) and 15 (19.5%) were included in the 7 valent vaccine. Four serotypes were the most frequently found in our strains: 7F (14.3%), 22F (11.7%), 3(10.4%) and 1 (6.5%). The thirteen valent conjugate vaccine confers efficacy against four of these serotypes (7F, 3, 19A and 1). The percentages of no susceptibility to penicillin and cefotaxime and resistant to erithromicin and levofloxain were 2.6%, 3.9%, 19.5% and 0%, respectively. Strains no susceptibility to penicillin or cefotaxime were included in the serotypes 14 and 6B. Thirty three percentage of erithromicin resistant strains were included in the serotype 19 A.

Conclusions: Serotypes most frequent found (7F, 22F, 3, 19A and 1), are included in the VP23 vaccine but no in VC7 vaccine. The thirteen valent conjugate vaccine confers efficacy against four of these serotypes (7F, 3, 19A and 1). The percentage of no susceptibility to b-lactams is low and it is in relation with the serotypes 14 and 6B. The erithromicin resistant is high and it is relation with the serotype 19A.

Session Details

Date: 10/04/2010
Time: 00:00-00:00
Session name: Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases
Location: Vienna, Austria, 10 - 13 April 2010
Presentation type:
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