Antimicrobial activity of herbal plants used in traditional medicine in Uzbekistan
Abstract number: R2176
Objectives: We chose 23 promising and widely used medicinal plants of Uzbekistan used traditionally by local inhabitants for treating conditions likely to be associated with microorganisms, and evaluated them for potential antimicrobial activity, in order to confirm their popular use and to detect new sources of antibacterial agents.
Methods: Plant material (20 g) was extracted with 80% methanol and the extracts were dissolved in 10% (v/v) solution of DMSO to create a concentration of 10 mg/ml of stock solution. The preliminary antimicrobial activity of the extracts was carried out by disc diffusion test. The plant extracts which showed inhibitory effect were further investigated for MIC using the broth microdilution method. For comparative purposes standard antibiotic discs viz., tetracycline (20 mg/disc), ampicillin (10 mg/disc), were used as positive and DMSO (10%, v/v) as the negative controls.
Results: Most of plant extracts were able to inhibit the growth of one or more of the tested strains at 2.5 mg/ml that corresponds to a concentration of 0.25% (Table 1). The plant species such Equisetum arvense, Polygonum aviculare and Limonium otolepis did not show any antibacterial activity against tested bacterial strains. The extract from Origanum tyttanthum showed the broadest spectrum of action against bacteria, inhibiting all of the strains tested with MICs ranging from 1.2 to 5 mg/ml, suggesting their potential as antimicrobial compounds. The extracts from aerial part of Betula verrucosa L. Calendula officinalis, Hypericum perforatum, Leonurus turkestanicus, Matricaria chamomilla, Tanacetum vulgare and Trifolium pretense were more active against Gram-positive, including S. aureus and MRSA (MICs ranging from 1.2 to 5 mg/ml) than against Gram-negative bacteria. They were also active against the fungus Candida albicans. Other plant species such Calendula officinalis, Hypericum perforatum, Melissa officinalis and Achillea millefolium have been reported and antimicrobial activities have been found. Among plant extracts only 5 species Matricaria chamomilla, Melissa officinalis, Hypericum perforatum, Tanacetum vulgare and Origanum tyttanthum inhibited the growth of H. pylori NCTC 12823.
Conclusion: The obtained results confirm the presence of antimicrobial principles in the examined herbal plants native habitats of Uzbekistan mainly against Gram-positive bacteria, which supports their traditional use as wound healing and skin infections in Central Asia.
Table 1. In vitro antimicrobial activity of plant extracts against human pathogenic bacteria (mg/ml)
|Session name:||Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Vienna, Austria, 10 - 13 April 2010|
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