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Antimicrobial activity of herbal plants used in traditional medicine in Uzbekistan

Abstract number: R2176

Objectives: We chose 23 promising and widely used medicinal plants of Uzbekistan used traditionally by local inhabitants for treating conditions likely to be associated with microorganisms, and evaluated them for potential antimicrobial activity, in order to confirm their popular use and to detect new sources of antibacterial agents.

Methods: Plant material (20 g) was extracted with 80% methanol and the extracts were dissolved in 10% (v/v) solution of DMSO to create a concentration of 10 mg/ml of stock solution. The preliminary antimicrobial activity of the extracts was carried out by disc diffusion test. The plant extracts which showed inhibitory effect were further investigated for MIC using the broth microdilution method. For comparative purposes standard antibiotic discs viz., tetracycline (20 mg/disc), ampicillin (10 mg/disc), were used as positive and DMSO (10%, v/v) as the negative controls.

Results: Most of plant extracts were able to inhibit the growth of one or more of the tested strains at 2.5 mg/ml that corresponds to a concentration of 0.25% (Table 1). The plant species such Equisetum arvense, Polygonum aviculare and Limonium otolepis did not show any antibacterial activity against tested bacterial strains. The extract from Origanum tyttanthum showed the broadest spectrum of action against bacteria, inhibiting all of the strains tested with MICs ranging from 1.2 to 5 mg/ml, suggesting their potential as antimicrobial compounds. The extracts from aerial part of Betula verrucosa L. Calendula officinalis, Hypericum perforatum, Leonurus turkestanicus, Matricaria chamomilla, Tanacetum vulgare and Trifolium pretense were more active against Gram-positive, including S. aureus and MRSA (MICs ranging from 1.2 to 5 mg/ml) than against Gram-negative bacteria. They were also active against the fungus Candida albicans. Other plant species such Calendula officinalis, Hypericum perforatum, Melissa officinalis and Achillea millefolium have been reported and antimicrobial activities have been found. Among plant extracts only 5 species Matricaria chamomilla, Melissa officinalis, Hypericum perforatum, Tanacetum vulgare and Origanum tyttanthum inhibited the growth of H. pylori NCTC 12823.

Conclusion: The obtained results confirm the presence of antimicrobial principles in the examined herbal plants native habitats of Uzbekistan mainly against Gram-positive bacteria, which supports their traditional use as wound healing and skin infections in Central Asia.

Table 1. In vitro antimicrobial activity of plant extracts against human pathogenic bacteria (mg/ml)

PlantKoKpEcPrCfAbPaSaEfEfcSeSarMlCaKaHp
Achillea millefolium L.nana55nana51.22.55na5nananana
Asparagus persicus Bakernananananananana52.55555nana
Betula verrucosa Ehrhnanananana2.51.21.21.22.5na2.52.51.2nana
Biden tripartite L.nanana5nana52.5na55nana52.5na
Calendula officinalis L.na2.52.5nanana551.22.52.51.21.22.5nana
Centaurea belangeriana Stapt.na55nananana55na5na5nanana
Dianthus tetralepis Nevskina55nananana52.52.555nananana
Erodium hoeffitianum CAMna2.55nananananana2.5nana2.52.5nana
Helichrysum arenarium L.nanananananananana1.25nanana1.2na
Hypericum perforatum L.nanananana552.52.51.2552.55na5
Leonurus turkestanicus L.nanana52.5na52.52.52.5555na5na
Matricaria chamomilla L.na5na5nanana51.21.252.52.52.51.25
Melissa officinalis L.5nana55nanana55na5nanana5
Origanum tyttanthum L.2.52.52.52.552.52.552.51.21.22.51.22.52.52.5
Peganum harmala L.2.552.5na51.22.52.51.252.52.5na2.55na
Plantago ovata L.na5na2.5na5na5551.25nanana
Scrophyllaria striata Boissna55nananananana2.552.5na2.55na
Tanacetum vulgare L.5na2.5nanana2.51.21.22.52.51.21.2na2.55
Thymus vulgaris L.nanananana2.5nana52.5na55nanana
Trifolium pretense L.nanana52.5na2.52.52.51.21.22.55na2.5na
na: not active; KoK. oxytoca 6653; KpK. pneumoniae 40602; EcE. coli NCTC9001; PrP. rettgeri NCIMB9570; CfC. freundii 82073; AbA. baumannii 60649; PaP. aeruginosa NCTC6749; SaS. aureus MRSA16; EfE. faecalis NCTC775; EfcE. faecium 8; SeS. epidermidis NCTC7944; SarS. aureus MRSA462; MlM. luteus; CaC. albicans NCVC1467; KaK. aerogenes NCTC8172; HpHelicobacter pylori NCTC12823.

Session Details

Date: 10/04/2010
Time: 00:00-00:00
Session name: Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Vienna, Austria, 10 - 13 April 2010
Presentation type:
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