Determination of Staphylococcus aureus and presence of staphylococcal enterotoxinA by molecular and microbiological methods in meatball meals cooked by two-bedded treatment institutions
Abstract number: P1955
Uyar F., Mirza C., Ruh E., Sener B., Alp A., Beyhan Y.
Objective: This study was planned and carried out for rapid determination of Staphylococcus aureus and Staphylococcal Enterotoxin A presence in meatball meals cooked in two different bedded treatment institutions (institution A and institution B) by using molecular and classical microbiological methods. Two genes, nucA and entA, were targeted in terms of S. aureus and Staphylococcal Enterotoxin A, respectively.
Methods: In this study, a total of 60 samples (30 samples from each institution) were included and the ability of real-time PCR technology for detection of nucA and entA genes was examined. FRET and TaqMan probes were designed for nucA and entA, respectively. Additionally, samples from meatball meals were also inoculated into sheep blood agars and S. aureus specific barid-parker media for further identification of S. aureus.
Results: By using real-time PCR, nucA and entA positive strains were detected in 23.1% and 16.5% of the samples from institution A and institution B, respectively. By the classical microbiological methods, S. aureus and S. aureus Enterotoxin A positive strains were detected in 16.5% and 6.6% of the samples collected from institution A and institution B, respectively. The results from molecular procedure were compared with those obtained from classical procedure and no statistical difference was reported (p > 0.05).
Conclusion: Our study shows that real-time PCR technology can succesfully be performed for rapid demonstration of S. aureus and enterotoxin A producing S. aureus strains in the food samples from food poisoning cases.
Our study also underlines the importance of the hygenic rules and sustainable training of personnel which should be ensured to prevent food poisoning cases in institutional food service systems.
|Session name:||Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Vienna, Austria, 10 - 13 April 2010|
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