Screening of the immunoprotective protein from the clinical strain of Mycobacterium tuberculosis
Abstract number: P1952
Suraiya S., Suppian R., Ashraful Haq J., Zaidah A.R., Musa M.
One third of the world's populations have been exposed to Mycobacterium tuberculosis. Approximately 2 million people died each year from the disease. Tuberculosis (TB) was predicted to be the largest single infectious cause of death between 1990 and 2020. In Malaysia, BCG vaccination is mandatory, given to all newborn and will be repeated at 12 years old if no BCG scar observed during routine school children medical examination. The tuberculosis protection by BCG is questionable, but thus far no new TB vaccine available in the market.
The objective of the study was to compare the immune reaction towards the BCG strain as compared to circulating strain of M. tuberculosis.
Materials and Methods: The BCG strain and the clinical strain of M tuberculosis were culture in the Middlebrooks supplemented with OADC. The whole protein lysate from both were extracted and the protein profiles were studied by the SDS-PAGE. The serum sample were taken from BCG vaccinated, healthy and The western blotting for detection of IgG were done from pool sera of BCG vaccinated healthy person and pool sera of confirmed TB patients.
Results: The whole protein lysate was successfully extracted from BCG strain and the clinical strain of M. tuberculosis. There is higher level of existing immune response against clinical strains of M tuberculosis as compared to immune reaction towards the BCG strains. There is also presence of a specific band around 90KD that only present in the healthy subject but not in the TB patients.
Conclusion: There is a potential protein present in the circulating clinical strain that might have an immunoprotective component. This specific band attracts special interest in the development of DNA based tuberculosis vaccine in the near future.
|Session name:||Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Vienna, Austria, 10 - 13 April 2010|
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