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Molecular epidemiology of enterovirus types causing aseptic meningitis in Greece, 20072009

Abstract number: P1860

Papadopoulou S., Apostolopoulou N., Piperaki E.T., Pitiriga V., Spanakis N., Tsakris A.

Background: Enteroviruses is considered the most common cause of viral meningitis. The detection and identification of the enteroviruses in such cases contributes to the proper use of antibiotics and to the assessment of their epidemiological profile. The aim of the present study was to detect and identify by molecular methods the circulating types of enteroviruses causing aseptic meningitis and encephalitis in Greece for the period 2007–2009.

Methods: One hundred and ten CSF specimens from patients-both children and adults-from 5 tetriary care hospitals in Athens with diagnosed or suspected aseptic meningitis, were examined in order to determine a possible enteroviral etiology.

RNA was extracted from CSF using the QuickGene-810 extraction system. RT-PCR targeting the 5-UTR of viral genome was used for detection of enteroviruses. RT-nested PCR targeting the VP1 gene, followed by sequencing, was then performed in order to determine the enterovirus serotype. The primers were supplied by Macrogen ltd and the RT-PCR reactions were carried out using the QIAGEN One-step RT-PCR kit.

Results: Fifty nine specimens (53.6%) were positive for the presence of enterovirus and 26 (44%) of the positive specimens were successfully typed. coxsackie B5 was determined as the predominant serotype (34.6%), followed by echovirus 30 (26.92%), echovirus 6, 9, 13 and coxsackie virus B4 (7.7% each), coxsackie A9 and echovirus 11 (3.8% each). Among the 59 patients 37 were male and 22 were female. The vast majority were children up to15 years old (96%) and only 4% were adults (>15 years). All, but one, patients who were found to be enterovirus positive showed abnormal CSF profile, with higher than normal white cell blood count (>5/mm3) and/or elevated protein level (>45 mg/dl) and all presented the typical clinical symptoms of aseptic meningitis.

Conclusions: Eight co-circulating enterovirus serotypes causing aseptic meningitis were identified. Coxsackie virus B5 was most frequently isolated, followed by echovirus 30. The prevalence of coxsackie virus B5 as well as the presence of echoviruses 6, 13, 11, 30 was also previously reported in Greece. However, echoviruses 15 and 4 that previously reported to cause outbreaks in Greece were not isolated in this study.

Session Details

Date: 10/04/2010
Time: 00:00-00:00
Session name: Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Vienna, Austria, 10 - 13 April 2010
Presentation type:
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