Antibodies to Bordetella pertussis antigens measured in saliva
Abstract number: P1757
Riffelmann M., Littmann M., Hülße C., Wirsing von Koenig C.
Objectives: IgG-anti-pertussis toxin (PT) antibodies used in pertussis serology are usually measured in serum samples. Measuring antibodies in saliva samples could offer an alternative.
Methods: 206 adolescent and adult patients with longer lasting coughs were recruited by 19 sentinel physicians (10 general practitioners, 9 paediatricians) in the Rostock (Germany) region between January 2008 and April 2009. Clinical symptoms were recorded. From every patient, a nasopharyngeal swab, a serum sample and a saliva sample was taken. Saliva samples were collected in a standardized manner by a commercially available device (OraSure®). Bordetella-DNA (B. pertussis and B. parapertussis) was detected by real-time PCR in nasopharyngeal swabs. The levels of serum IgG and IgA, and the levels of saliva IgG and IgA were measured by rate nephelometry. IgG- and IgA-antibodies to PT and filamentous hemagglutinin (FHA) were quantified by a standardized ELISA in serum and saliva samples. In serum samples, IgG-anti-PT levels of 100 IU/ml were regarded as indicative of recent contact, and IgG-anti-PT levels of 40 IU/ml were interpretede as not indicative of a recent contact.
Results: In a total of 45 patients (18%) a recent contact to Bordetella was substantiated either by serum antibodies alone or also by PCR (n = 12). PCR was more often positive in adolescent patients. Antibodies in saliva could be measured by the standardized ELISA procedure used for serum samples. The antibodies measured in saliva correlated well with the serum concentration, when the total IgG / IgA content of the saliva samples was taken into account. Similar to serum, IgG anti-PT was found to be more often indicative of recent contact than IgA-anti-PT.
Conclusions: Measuring IgG-and IgA-anti-PT antibodies in saliva samples taken with a standardized device and correlated to the total IgG/IgA content of the saliva could offer an alternative to measuring anti-pertussis antibodies in serum samples.
|Session name:||Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Vienna, Austria, 10 - 13 April 2010|
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