Association of plasma levels of serum amyloid in kidney transplant patients with cytomegalovirus infection
Abstract number: P1752
Steininger C., Höpler W., Kerschner H., Popow-Kraupp T., Kundi M., Kletzmayr J., Schmaldienst S., Graninger W.
Objectives: Cost-effective pre-emptive therapy of cytomegalovirus (CMV) infection in solid-organ transplantation requires frequent screening of patients for active CMV infection with the use of inexpensive rapid diagnostic assays. Serum amyloid A (SAA) is an acute phase protein that was used successfully to identify in paediatric patients acute respiratory virus infections or in HIV-infected patients CMV infections. We evaluated the usefulness of measurement of SAA and high-sensitivity C-reactive protein (hs-CRP) levels in plasma for the identification of kidney transplant patients with CMV infection.
Methods: Plasma samples (n = 132) were collected from 23 CMV-seronegative patients who either received an organ from a CMV-seropositive (D+/R-, n = 16) or a CMV-seronegative donor (D-/R-, n = 7). All samples were screened for the presence of CMV-DNA and increased levels of SAA or hs-CRP.
Results: Primary CMV infection was diagnosed in 16 (100%) of the D+/R- patients and in one (14%) of the D-/R- patients. CMV-DNA was detected in 70 of the 132 (53%) samples and increased SAA levels were detected in 96% of CMV-DNA-positive samples. Levels of SAA correlated well with those of hs-CRP (P = 0.001). However, levels of SAA were increased in almost all available samples (125/132, 95%) and overall, increased levels of SAA were not associated with presence of CMV-DNA (P = 0.430). Some samples negative for CMV-DNA but positive for SAA were collected concomitantly with manifestations of other acute inflammatory disorders including acute kidney rejection (n = 7), or respiratory, gastrointestinal, or urinary tract infections (n = 8).
|Session name:||Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Vienna, Austria, 10 - 13 April 2010|
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