Molecular typing of methicillin-resistant Staphylococcus aureus in animal origin foods
Abstract number: P1700
Salehipour Z., Soltan Dallal M.M., Eshraghi S., Zahraei Salehi T., Rahimi Forushani A., Abedi Mohtasab T.P., Fallah Mehrabadi J.
Objective: Methicillin-resistant staphylococcus aureus (MRSA) strains are common pathogens in serious infections and nosocomial outbreaks. These strains might be transmitted to humans via consumption of animal origin foods. Aim of this study is prevalence and molecular characterization of these strains in different foods.
Methods: 913 food samples of animal origin were collected from July 2006 to November 2007 and cultured on Baird Parker agar. All suspected colonies were characterized by biochemical tests. presence of mecA gene was confirmed by PCR method. MRSA isolates were evaluated by the disk diffusion method for different antimicrobial agents along with minimum inhibitory concentration (MIC) determination of oxacillin. Presence of tst gene in MRSA isolates was assessed via PCR and reverse passive latex agglutination test and followed by biotyping and spa typing.
Results: Among 93 S. aureus isolates, five (5.37%) harbored the mecA gene. 60% of the five MRSA isolates were resistant to tetracycline, while 40% were resistant to Ceftriaxone. Four out of five MRSA isolates (80%) produced SEs and TSST1. Three MRSA isolates belonged to human biotype, whereas the remaining two isolates belonged to non-host-specific (NHS) biotype. Amplification of spa gene showed three different fragments. Applying HindIII and HaeII, 4 and 3 distinct RFLP patterns were yielded, respectively.
Discussion: In present study, prevalence of MRSA strains in foods is worrying relatively. Bacterial strain typing for MRSA can be considered a tool of importance to investigate suspected outbreaks together with their control and surveillance, and PCR-RFLP is a reliable method for typing.
|Session name:||Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Vienna, Austria, 10 - 13 April 2010|
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