Changing patterns of antibiotic resistance and serotype distribution in groupB streptococcus isolated in a maternity hospital in Kuwait
Abstract number: P1681
Boswihi S., Al-Sweih N., Udo E.
Objectives: Group B streptococcus (GBS) is a common cause of infections in neonates and pregnant women. Although still susceptible to penicillin, there are reports of reduced susceptibility to penicillin in some GBS isolates. In this study GBS isolates obtained from patients at the Maternity hospital in Kuwait were studied for their serotypes and susceptibility to antibiotics, and the results were compared to those of a previous study conducted in 2001.
Methods: A total of 154 GBS isolates were collected from 1 July to 31 October 2007 from HVS, urine, blood and miscellaneous sources. All isolates were serotyped using latex agglutination test. Antibiotic susceptibility testing was performed by the disk diffusion method. MIC for penicillin, erythromycin and clindamycin was performed by E-test or agar dilution methods.
Results: Most of the isolates belonged to serotype V (38.3%), serotype III (19.5%), serotype Ia (10.4%), serotype II (10.4%). Seventeen isolates (11.0%) were nontypeable. Eighteen (11.68%) and 10 (6.5%) isolates were resistant to erythromycin (MIC: >1 mg/L) and clindamycin (MIC: >2 mg/L), respectively. They were susceptible to penicillin. However MIC determination showed that 31.2% of them had elevated penicillin MIC values of 0.064 mg/L and 13.6% had MIC of - 0.094 mg/L. Eight isolates (5.2%) expressed reduced susceptibility to penicillin (MIC: 0.1250.19 mg/Ll).
Conclusion: The result showed that serotype V detected in 38.3% of the isolates became the dominant serotype in 2007 whereas serotype III was the dominant serotype in 2001. The proportion of GBS isolates resistant to erythromycin and clindamycin increased from 0.7% and 1.7% in 2001 to 11.6% and 6.5% in 2007. There was a significant shift in the number of isolates expressing penicillin MIC of 0.064 mg/L from 4.4% in 2001 to 44.8% in 2007. Furthermore, whereas none of the 2001 isolates had MIC 0.1250.19 mg/L, this was detected in 5.2% of the 2007 isolates. The result demonstrated a trend towards increasing antibiotic resistance in GBS isolates in a Kuwait hospital.
|Session name:||Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Vienna, Austria, 10 - 13 April 2010|
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