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Antimicrobial resistance in faecal Escherichia coli isolated from calves

Abstract number: P1643

Kmet V., Bujnakova D., Novak S., Kmetova M.

Objectives: Commensal animal Escherichia coli may act as reservoir of antibiotic resistance genes (blactamase and quinolone) and virulence factors (verotoxins) for human population. Animal Escherichia coli fluoroquinolone resistance is increasing (Slovak Antimicrobial Resistance Veterinary Database, http://www2.saske.sk/atbres/). The aim of study was to characterize antibiotic resistance of faecal Escherichia coli, resistance mechanisms and verotoxin production.

Methods: During 2009 year a 123 faecal Escherichia coli isolates from calves from various regions in Slovakia were isolated and analyzed. For phenotypic antibiotic resistance determination, CLSI (2008) criteria were used. Verotoxin genes (vt1 and vt2), ESBL CTX-M, plasmid quinolone resistance (qnrA, B, S), integron 1, replicon typing of plasmids and phylogenetic analysis were determined by PCR.

Results: The most frequently occurences were resistance to tetracycline (86%), streptomycin (78%) and ampicillin (79%). Among tested Escherichia coli isolates also high resistance rates to neomycin (50%), chloramphenicol (59%) and florfenicol (30%), cotrimoxazole (49%) and spectinomycine (21%) could be recorded. Resistance to ampicillin+sulbactam (10.6%), ceftiofur (1.6%), gentamicin (14%) were lower. The high level of fluoroquinolone MICs (CIP geqslant R: gt-or-equal, slanted4 mg/L and ENR geqslant R: gt-or-equal, slanted16 mg/L) reached 42%, however plasmid quionolone resistance was not detected. Majority of strains contained vt1 or vt2 genes, integron 1 and plasmid replicon types B/O, FIC, FIB or F1B. Strains belonged to pathogen phylogenetic group B2 a D and commensal group A and B1 also. Only one strain with ESBL CTX-M1 was detected in Escherichia coli from one farm.

Conclusions:

1The high level of fluoroquinolone MICs in Escherichia coli was recorded and food animals could be a reservoir of quinolone resistance for human population.

2ESBL CTX-M1 was detected in Escherichia coli only from one farm.

3Majority of faecal Escherichia coli strains were verotoxigenic and belonged to pathogen and commensal phylogenetic groups.

Session Details

Date: 10/04/2010
Time: 00:00-00:00
Session name: Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Vienna, Austria, 10 - 13 April 2010
Presentation type:
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