The emergence of the major European clones of carbapenem-resistant Acinetobacter baumannii in Kuwait

Abstract number: P1395

Al-Hasan A., Hamouda A., Amyes S.G.

Objectives:Acinetobacter baumannii is an increasingly important nosocomial pathogen because of its ease of transmission and ability to become multidrug resistant. It has become a particular problem in Kuwaiti hospitals and this study aimed to examine the genotypic changes in the organism as it spread through one hospital.

Methods: A total of 88 Acinetobacter baumannii samples were collected from the Mubarak Al-Kabeer Hospital, over a three year period, 2006–2008, and they were identified phenotypically, by Vitek-2 systems, and then genotypically, by PCR amplification of blaOXA-51-like gene. The resistance to the carbapenems, Imipenem and Meropenem, was identified by use of the Minimal Inhibitory Concentration (MIC) test. Pulsed Field Gel Electrophoresis (PFGE) was used to type the strains and classify them into clonal groups. With this information, a dendogram showing the resistance profile and clonal relationship of all the isolates was constructed. Gene Sequencing was used to identify the blaOXA-51-like gene types of each of the isolates.

Results: All 88 isolates were identified as Acinetobacter baumannii by Vitek-2 system and were shown to carry a blaOXA-51-like gene. Resistance to Imipenem was found in 30% of the isolates, whereas resistance to Meropenem was found in 24% of the isolates. Overall carbapenem resistance was observed in 32% of the total isolates, with a slight increase in resistance of isolated over the 3 years of collection. In all, there were 10 different blaOXA-51-like genes identified. The sequences of these genes suggested there was some degree of real-time evolution of the blaOXA-51-like genes during the study period. There were four main clonal clusters. There were three main European clones (blaOXA-66, blaOXA-69, and blaOXA-71) plus a new clone-with blaOXA-51-like genes with sequences clustered around the blaOXA-98 gene.

Conclusion: This study has shown four major clones were found in the hospital during the study period, three closely associated with those found in Europe and elsewhere and one new clone, containing a blaOXA-98-like gene that appears to be more prevalent in this part of Asia.

Session Details

Date: 10/04/2010
Time: 00:00-00:00
Session name: Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases
Location: Vienna, Austria, 10 - 13 April 2010
Presentation type:
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