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Analysis of carbapenem resistance genes in Acinetobacter baumannii isolates from Kuwait hospitals

Abstract number: P1302

Al-Sweih N., Al-Hubail M., Rotimi V.O.

Objectives: This study was designed to investigate the molecular epidemiology and genetic basis of the carbapenem resistance in clinical isolates of Acinetobacter baumannii obtained from all government hospitals in Kuwait.

Methods: A total of 250 clinical isolates were collected from 8 hospitals. Their susceptibility to 18 antibiotics was determined by E test method. Carbapenems resistant isolates were screened for phenotypic MBL production by disk approximation test (DAT) and MBL E test. Genetic characterization of the resistant mechanisms was performed by PCR. Their clonal relatedness was assessed by PFGE.

Results: All the isolates were multidrug resistant. Of the 250 isolates, 93 (37.2%) were resistant to carbapenems. The prevalence of MBL-producing isolates was 74.2% and 57% by DAT and MBL Etest, respectively. Sixty-five (69.9%) of the carbapenem-resistant isolates were positive for one or more resistance genes; 37 were positive for blaIMP-1, 17 blaVIM-1, 43 blaVIM2, 24 blaSPM-1, 32 blaOXA-23, 1 blaOXA-24. PFGE demonstrated widespread clones of similar strains in different hospitals and a cluster of 3 clones found only in one particular hospital.

Conclusion: Resistance clinical isolates to carbapenem has reached unacceptable levels in Kuwait and MBLs, as well as oxacilinases, are highly prevalent among A. baumannii in our hospitals.

Acknowledgment: This work was supported by a Kuwait University Research Grant no. YM 01/08 which is gratefully acknowledged.

Session Details

Date: 10/04/2010
Time: 00:00-00:00
Session name: Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Vienna, Austria, 10 - 13 April 2010
Presentation type:
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