Dissemination of a clone KPC-3-producing Klebsiella pneumoniae in hospitals, Bogota, Colombia
Abstract number: P1281
Saavedra S.Y., Alvarez C.A., Cuervo S.I., Olarte N., Escobar J.A., Leal A.L., Castillo Londoño J.S.
Objective: Typing molecular from isolates carbapenem-resistant Klebsiella pneumonia and to detected microbiological and molecular ocurrence from carbapenemases.
Methods: Fifty-eight isolates carbapenem-resistant K. pneumoniae recovered from patients in six hospitals of Bogota were collected from 2008 to 2009. The identification and the susceptibility testing were performed with automated system; imipenem, meropenem and ertapenem susceptibilities were determined by disk diffusion and the carbapenemases production was confirmed with the modified Hodge test. The detection of genes encoding metallo and KPC-type carbapenemases was performed by PCR using specific primers for blaIMP, blaVIM and blaKPC genes. Amplification products were analyzed by restriction disgestion of the amplicom with the enzymes BstNI and RsaI and confirmed by sequentiation. The characterisation molecular by assessment the relatedness of the isolates was realised by ERIC-PCR, the cluster analysis was realised with Fingerprinting II software (Bio-Rad).
Results: All Isolates were resistant to ertapenem, ceftriaxone, ceftazidime, cefotaxime, eleven isolates were susceptible to imipenem and seven to meropenem. All isolates were phenotypically identified as possible carbapenemases producers and the presence of blaKPC gene was confirmed by PCR. Sequencing and restriction test confirmed the presence of KPC-3 in all isolates. Typing results showed presence of a clone predominant conformed by fifty-seven isolates from five hospitals and a fingerprint unique genomic.
Conclusions: Our results indicate the ocurrence of a clone KPC-3 producing K. pneumoniae in five hospitals from Bogota. This is the first report of dissemination of KPC-3 in hospitals from Colombia.
|Session name:||Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Vienna, Austria, 10 - 13 April 2010|
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