High diversity of HHV8 molecular subtypes in AIDS-KS Cuban individuals and its relation with HHV8 load: evidence of subtypeB expansion
Abstract number: P1204
Martínez P., Kouri V., Aleman Y., Blanco O., Capó V., Rodríguez M.E., Dovigny M., Cardellá L., Gala A., Jiménez N., Luzardo C., Correa C., Pérez L., Alvarez A., Hengge U.
Objectives: With the aim of investigate the distribution of KSHV genotypes and its association with diseases pathogenesis, 30 Cuban AIDS-KS patients diagnosed between 2005 and 2007 were studied.
Methods: Samples of plasma, saliva, peripheral blood mononuclear cells (PBMC) and affected tissue were obtained for KSHV quantification. KSHV genotyping was performed by the nucleotide sequencing of the variable ORFK1 gene from tissue samples. Amino acid sequences of this gene were used to construct a neighbour-joining phylogenetic tree. The median KSHV viral load (copies/100ng DNA) were 39, 151, 830603 and 143 in plasma, saliva, tissue and PBMC, respectively; being the viral load from affected tissue and saliva significantly higher in comparison to the other samples.
Results: Phylogenetic characterization of Cuban specimens showed a wide variety of subtypes [(A: n = 6 (20%), five being A5 (16.7%); C: n = 6 (20%); B: n = 10 (33.3%); and subtype E: n = 3 (10%)], showing an increasing tendency in the incidence of African genotypes (B and A5) among the new AIDS-KS cases in comparison with previous reported Cuban KSHV sequences. Interestingly, detection of type B KSHV strains was associated with higher viral load in tissue and saliva samples (p < 0.01).
Conclusion: The present result suggests that KSHV subtype B might have evolve to more efficient transmission over other subtypes among Cuban AIDS-KS patients, replacing KSHV A subtype that used to be more frequent before 2006.
|Session name:||Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Vienna, Austria, 10 - 13 April 2010|
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