Detection and typing of human papillomaviruses in malignant, dysplastic, non-dysplastic and normal oral epithelium by nested polymerase chain reaction
Abstract number: P1182
Blioumi E., Chatzidimitriou D., Pazartzi C., Katopodi T., Tzimagiorgis G., Melidou A., Emmanouil-Nikoloussi E., Markopoulos A., Lazaridis N., Skouras Z., Diza E., Antoniades D.
Introduction: Studies on human papillomaviruses (HPV) participation in initiation and progression of oral neoplasia have generated conflicting results. Specifically, HPV infection in oral carcinomas ranges from 0%-100% and in the normal oral cavity from 060%.
Aim: This study aims at the detection of the HPV DNA in oral carcinomas, dysplastic leukoplakias, non dysplastic leukoplakias, fibromas and normal mucosa by nested polymerase chain reaction (nested-PCR) and the correlation of HPV presence with other factors obtained from the patient's records, such as histologic diagnosis, gender, age, anatomic location, smoking and alcohol drinking.
Material and Methods: 258 formalin-fixed paraffin-embedded tissues (63 carcinomas, 57 leukoplakias with dysplasia, 53 leukoplakia without dysplasia, 50 fibromas and 35 normal tissues) were deparaffinised, After DNA extraction, a nPCR was performed using primers targeting the HPV L1 gene; in the first step MY09/11 and in the second step GP5+/GP6+. Positive PCR products were sequenced for further HPV typing.
Results: HPV was detected in 22.2% of carcinomas, 31.5% of dyspalsias, 35.8% of non-dysplastic leukoplakias, 40% of fibromas and 40% of normal mucosas. HPV 16 was detected in 90% of positive samples. Statistical analysis with X2 and pearson correlation test shows significant negative correlation between HPV prevalence and age. No significant correlation was detected between HPV prevalence and gender, histological diagnosis, histological grading, location, smoking or alcohol drinking.
Conclusions: HPV was detected in all study groups, though in normal tissues more often than dysplastic or malignant. This fact does not exclude nor confirm the participation of HPV with the early stages of oral carcinogenesis.
|Session name:||Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Vienna, Austria, 10 - 13 April 2010|
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