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Cytomegalovirus infection in neonatal intensive care units

Abstract number: P1172

Senok A., Al-Khawaja S., Ismaeel A., Botta G.

Background: Human cytomegalovirus (HCMV) infections are among the most prevalent viral infections worldwide with rates of congenital infections varying in different populations. This study was to determine the prevalence of congenital and perinatal HCMV infections among newborns in two major neonatal intensive care units (NICU) in Bahrain.

Methods: Preterm and term babies admitted to the NICU during the study period (February-June 2006) as well as their mothers were included in the study. During the first six weeks of life, urine and saliva was obtained from the babies weekly and serial breast milk samples were obtained from the mothers. Maternal serum HCMV IgG was measured. Virus isolation and detection was by shell vial culture and nested PCR. HCMV infection was diagnosed in infants with virus excretion detected by both methods on at least two occasions or in two different specimen types.

Results: A total of 100 newborns (84 preterm; 16 term) and their mothers were included in the study. Maternal HCMV IgG-seropositivity was 100%. Congenital HCMV infection was confirmed in 3 newborns (preterm 2/84; 1.9%; term 1/16; 6.3%). HCMV DNA was detected in the breast milk samples obtained during the first 10days postpartum from mothers whose babies had congenital HCMV. Among breast milk samples collected 4–6weeks post-partum 22.5% were HCMV DNA positive. It was determined that five pre-term babies acquired perinatal infections via HCMV positive breast milk at 4–6weeks postpartum. There were no significant difference in the detection of HCMV using shell vial culture versus nested PCR method.

Conclusion: Our findings suggest that neonatal ICU care should include neonatal HCMV screening to detect the infection during early postnatal period. Shell vial culture and PCR amplification represent rapid and sensitive methods can be used for diagnosis of suspected congenital HCMV infections.

Session Details

Date: 10/04/2010
Time: 00:00-00:00
Session name: Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Vienna, Austria, 10 - 13 April 2010
Presentation type:
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