Investigation of the frequency of pandemic influenzaA 2009 H1N1 virus and other respiratory viruses in patients with acute respiratory tract infections
Abstract number: P1099
Cicek C., Pullukcu H., Saz E.U., Cok G., Arda B., Bacakoglu F.
Objectives: The aim of this study is to establish the frequency of respiratory viruses (RSV, influenza viruses type A and B, parainfluenza viruses type 1, 2, and 3, and adenovirus) and pandemic influenza A 2009 H1N1 virus in patients with acute respiratory tract infections in Izmir since January 2009.
Methods: Nasopharyngeal swab specimens were collected from 480 [251 (52.3%) male, 229 (47.7%) female] patients with acute respiratory tract infections between January 1st and November 17th 2009. The age range of patients is between 6 days to 82 years (median: 15 years). Direct immunofluorescent antibody (DFA) test and shell vial cell culture method were performed when all the specimens arrived at the laboratory. Cytocentrifuged specimens were stained with Respiratory DFA Viral Screening & Identification Kit (Light Diagnostics, Millipore, USA) according to the manufacturer's instructions. For the recovery of respiratory viruses were used HEp-2 (RSV), A-549 (adenovirus), Vero (parainfluenza viruses type 1, 2, and 3), and MDCK (influenza viruses type A and B) cells line and shell vial cell culture method were used. Coverslips were stained with a fluorescein isothiocynate labelled monoclonal antibody specific for each virus (Light Diagnostics, Millipore, USA) according to the manufacturer's instructions. Pandemic influenza A 2009 H1N1 virus was detected by real time RT PCR assay (Influenza A H1N1 primer and probe set, SuperScript III Platinum® One-Step qRT-PCR System, Invitrogen, USA) by using ABI 7500 system.
Results: Of the 480 patients, 141 (29.4%) were positive for respiratory viruses by using the combination of DFA and shell vial cell culture method and five of these patients were infected by two different viruses [18 (12.3%) RSV, 99 (67.8%) influenza type A, 5 (3.4%) influenza type B, 9 (6.2%) parainfluenza viruses, and 15 (10.3%) adenovirus]. Of 99 influenza A viruses, 94 (94.9%) were typed as pandemic influenza A 2009 H1N1 virus.
Conclusion: Pandemic influenza A 2009 H1N1 virus was not very common until the second week of November in Izmir. This may be due to the temperate climatic conditions during this period (20240C) in Izmir. Two-thirds of pandemic influenza A 2009 H1N1 virus was detected between November 9th and 17th due to cold weather. The frequency of the other respiratory viruses decreased after September. Except for influenza A virus, no other respiratory viruses have been detected in patients since the first of November.
|Session name:||Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Vienna, Austria, 10 - 13 April 2010|
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