Presence of several respiratory viruses in samples from patients with suspected flu A/H1N1 infection

Abstract number: P1094

Álvarez-Buylla A., Culebras E., Betriu C., Picazo J.

Objectives: Infections caused by the influenza virus have a significant repercussion on modern society and causes particular concern. Since many of the signs and symptoms of respiratory virus infections are similar, other respiratory virus pathogens can be overlooked when focusing only on FluA. The aim of this study was to determine the presence of different respiratory viruses in both positive and negative pandemic influenza A samples.

Materials and Methods: A total of 60 respiratory specimens collected from patients with influenza-like illness had been selected for the study and previously tested by real time PCR. Specimens included 30 positive and 30 negative for pandemic FluA (15+15 samples from June and 15+15 samples from October).

Samples were tested using commercial kit (CLART PneumoVir. Genomica®) that employ a combination of two multiplex RT-PCR followed by primer extension and microarray hybridization. This assay can detect adenovirus; bocavirus, coronavirus; enterovirus (echovirus); rhinoviruses (HRV); influenza viruses A (seasonal), B and C; human metapneumoviruses (HMPV) A and B, human respiratory syncytial viruses (HRSV) A and B; and parainfluenza viruses (PIV) of types 1, 2, 3 and 4.

Results: No other respiratory viruses were detected in pandemic FluA positive samples from June, whereas six positive specimens from October were identified as dual infections. These dual infections included: one type 4 PIV, one HRV, one type 1 PIV, one RSV and two seasonal FluA. In pandemic FluA negative samples 7 out of 15 specimens from June reveal the presence of another respiratory virus (two samples with adenovirus, 2 samples coronavirus, one type 4 PIV, one enterovirus and one FluC). Only three of the negative pandemic FluA samples analyzed from October were positive in our study (HRV the three specimens).

Conclusions: Different respiratory viruses are present in samples obtained from patients with influenza-like illness. Detection of these other respiratory viral etiologic agents can be difficult in cases in which the causal agent was not suspected and a specific test was not demanded by the clinicians. The circulation of different respiratory viruses during the same period of the year makes very complex to elucidate their individual contributions to the global respiratory disease.

Session Details

Date: 10/04/2010
Time: 00:00-00:00
Session name: Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases
Location: Vienna, Austria, 10 - 13 April 2010
Presentation type:
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