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Quantitative HHV-6B antigenemia test for the monitoring of transplant patients

Abstract number: P1088

Loginov R., Karlsson T., Höckerstedt K., Ablashi D., Lautenschlager I.

Objectives: Human herpesvirus-6 (HHV-6) infection, mostly caused by the variant B, is common after solid organ transplantation, as previously demonstrated by antigenemia test. Here we report a new modified method using a HHV-6B glycoprotein IgG-antibody, OHV-3, and attempt to quantify the HHV-6 antigenemia after liver transplantation. Twenty-four adult liver transplant recipients were frequently monitored by the qualitative HHV-6 antigenemia test, which detects the HHV-6B virion protein in peripheral blood mononuclear cells (PBMC).

Methods: HHV-6B antigens were now demonstrated using a glycoprotein OHV-3 IgG-antibody in the immunoperoxidase staining from the same specimens and quantified as positive cells/10,000 PBMC. The results were confirmed and quantified by DNA-hybridization in situ using biotinylated oligoprobe. Altogether 206 blood specimens were analyzed.

Results: During the first six months, HHV-6 antigenemia was detected in 17/24 (71%) recipients by using HHV-6B virion antibody. All, except one patient with a faint positivity, demonstrated positive findings also with the OHV-3 antibody. Altogether, 37% (77/206) of specimens were positive with the virion antibody and 39% (78/201) by the OHV-3 antibody. The peak number of OHV-3 positive cells in the PBMC`s varied from 5 to 750/10,000 (mean 140/10,000).

Conclusions: The OHV-3 antibody was useful to quantify the HHV-6B antigenemia. The findings of the HHV-6B quantitative antigenemia using the OHV-3 antibody correlated well with the previous qualitative HHV-6 antigenemia assay, and can be used as a quantitative method in the monitoring of HHV-6 in transplant patients.

Session Details

Date: 10/04/2010
Time: 00:00-00:00
Session name: Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Vienna, Austria, 10 - 13 April 2010
Presentation type:
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