Genetic fingerprinting of MRSA from Abu Dhabi
Abstract number: P1060
Weber S., Ehricht R., Slickers P., Abdel-Wareth L., Donnelly G., Pitout M., Monecke S.
Methicillin-resistant Staphylococcus aureus (MRSA) have become a global problem. However, for many regions of the world only few, if any, data on the actual distribution and abundance of various epidemic or pandemic strains are available. This includes many countries of the Middle East. In order to obtain such typing data, clinical isolates from Sheik Khalifa Medical City, Abu Dhabi, have been characterised using diagnostic DNA-microarrays.
Out of 62 MRSA isolates tested, two carried SCCmec elements of type III. The most common SCCmec element was type IV which was present in a total of 54 isolates. SCCmec elements of type V were found in six isolates. SCCmec elements of types I and II were not detected. Common resistance determinants included blaZ, ermC and genes encoding aminoglycoside-modifying enzymes. Mupirocin resistance was rare with mupR being detected in a single isolate. PVL genes were present in as much as 26 isolates.
Based on array hybridisation profiles, eighteen strains could be distinguished which belonged to eleven clonal complexes. The most common clonal complex was CC22 (21 isolates), followed by CC8 (nine isolates, including USA300, ST72 and ST239 strains) and CC80 (seven isolates). Several unusual MRSA strains (such as ST6-MRSA-IV, CC361-MRSA-V or ST573/772-MRSA-V) were also found.
Generally, a high diversity of different strains as well as a rather high rate of PVL carriage were noted among isolates from Abu Dhabi. These results warrant further investigations on the epidemiology of MRSA in this region.
|Session name:||Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Vienna, Austria, 10 - 13 April 2010|
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