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Antifungal susceptibility patterns of Candida isolates originated from invasive candidiasis

Abstract number: P850

Objectives: The aim of our study was to evaluate the antifungal profiles, against commonly used and newer antifungal agents of Candida isolates, causing invasive candidiasis.

Methods: During one year period (11/2008–10/2009) we examined forty three Candida isolates responsible for thirty seven distinct cases of candidaemia and six other invasive candidiasis. The latter isolates derived from surgical wounds (n = 3), lower respiratory tract specimens (n = 2) and normally sterile samples (n = 1, ascitic fluid) each of them represented a unique infectious episode. The fungal identification procedure to species level included germ tube production and the use of the automated compact system VITEK 2 (Biomerieux). The antifungal susceptibility testing was performed by using E-test strips (Biomerieux) and RPMI 1640 agar with 2% glucose according to the manufacturer's instructions. MIC was evaluated according to CLSI criteria for 5-flucytocine (FC), fluconazole (FL), voriconazole (VO), micafungin (MCF) and anidulafungin (AND) while a provisional susceptibility breakpoint of leqslant R: less-than-or-eq, slant1 mg/L was used for amphotericin B (AP) and posaconazole (POS).

Results:C. parapsilosis was the predominant species (n = 31, 72%), followed by C. albicans (n = 12, 28%). All isolates were inhibited at MICs leqslant R: less-than-or-eq, slant1 mg/L of amphotericin B, posaconazole and micafungin. Only 5 out of 31 (17%) C. parapsilosis isolates were susceptible to AND while all C. albicans isolates were susceptible to the same agent. 37 isolates were examined and found susceptible to the new echinocandins AND, MCF, and to triazoles POS and VO. The susceptibility percentage of the examined C. parapsilosis / C. albicans isolates to the rest antifungal agents, was 88.4% / 83.7% for FC against C. parapsilosis / C. albicans, and 34.9% / 30.2% for FL against the same isolates, respectively.

Conclusion:C. parapsilosis prevails among the isolates responsible to invasive candidiasis, in our hospital. The more recent antifungal agents POS and MCF as well as AP, VO were active against all Candida isolates tested, FC possessed a satisfactory antifungal activity and AND was active against all the C. albicans isolates. The low degree of susceptibility to FL and AND observed against some Candida isolates highlights the necessity of administrating antifungal agents in accordance to the susceptibility test of the responsible isolates.

Session Details

Date: 10/04/2010
Time: 00:00-00:00
Session name: Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Vienna, Austria, 10 - 13 April 2010
Presentation type:
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