Aetiology of eye infections in hospitalized ophthalmological patients

Abstract number: O366

Wroblewska M.M., Broniek G.

Objectives: Ophthalmological patients are prone to nosocomial infections due to advanced age and common underlying medical conditions. Eye infections caused by multi-drug resistant (MDR) pathogens may further contribute to loss of vision in already visually disabled persons. Antimicrobial therapy is often difficult due to unsatisfactory penetration of many agents into the eye. Empiric therapy should be based on data comprising most prevalent pathogens, including MDR pathogens.

Methods: Specimens for bacteriological and fungal culture were obtained from patients hospitalized in ophthalmological hospital (2005–2009). Samples were cultured and isolates identified according to standard microbiological techniques.

Results: In total 1662 specimens were cultured, including swabs from conjunctiva, cornea, throat, nose, wounds and cornea conservation medium. The samples yielded 328 isolates, comprising 293 strains of bacteria and 7 strains of yeast-like fungi. Among bacteria predominated staphylococci – 197/293 (67.23%), followed by enteric rods – 36/293 (12.29%), non-fermenting rods – 20/293 (6.87%) and streptococci – 19 (6.48%). Among alarm pathogens there were methicillin-resistant S. aureus (MRSA) strains (4/50, 8.00%), high-level aminoglycoside resistant (HLAR) strains of enterococci (1/8, 12.50%), extended-spectrum b-lactamase producing – ESBL(+) enteric rods (2/36, 5.56%) and ESBL(+) Gram-negative nonfermenting rods (4/20, 20.00%). Methicillin-resistant coagulase-negative staphylococci (MRCNS) comprised 28/147 (19.05%) strains. Among all cultured microbial isolates there were 7/300 (2.33%) strains of fungi. Predominated Candida parapsilosis (4/7 strains), followed by C. albicans (2/7) and C. glabrata (1/7).

Conclusion: In ophthalmological specimens predominated Gram-positive cocci, mainly staphylococci. The presence of MRCNS (19%) may be important in therapy of biofilm-associated implant infections. In this study there was a high percentage (20%) of ESBL(+) Gram-negative nonfermenting rods. Among yeast-like fungi Candida parapsilosis was more often isolated than C. albicans. This is clinically relevant as C. parapsilosis tends to form a biofilm on artificial materials, increasingly used in ophthalmic surgery.

Session Details

Date: 10/04/2010
Time: 00:00-00:00
Session name: Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases
Location: Vienna, Austria, 10 - 13 April 2010
Presentation type:
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