Mycobacterium in paediatric patients during a 2year period in a health district of Madrid, Spain

Abstract number: O138

Somodevilla Á., Domingo D., Agudo S., Hernández B., Alonso M., Lopez-Brea M.

Objective: The aim of this study is to describe the prevalence of tuberculosis and non-tuberculosis mycobacteria isolates in paediatric patients during a two year period (November 2007 to November 2009) in a health district of Madrid (Spain).

Methods: Four hundred and ninety eight specimens from paediatric patients were proceseed for mycobacterial study. All of them were prepared for auramine stain and decontaminated by NaOH-N-acetylcysteine method. When the specimen was a gastric acid, it was neutralizated before with bicarbonate of sodium. After decontamination each specimen was cultured in liquid and solid medium (MIGT and Coletsos, respectively). If growth was detected a Zielh-Neelsen stain was done. Accuprobe kit (Gen-Probe) was used to identificate Mycobacterium tuberculosis Complex or Mycobacterium avium Complex strains. Other Non Tuberculosis Mycobacteria (NTM) were identified by solid hybridization using GenoType Mycobacterium kit (Hain Lifescience).

Results: Thirty two (6.42%) out of 498 specimens received were positive: 17 Mycobacterium tuberculosis Complex (53.13%), 9 Mycobacterium abscessus (28.12%), 4 Mycobacterium avium Complex (12.5%) and 2 Mycobacterium lentiflavum (6.25%).

They were obtained from different specimens from 22 patients:

M. tuberculosis Complex: 14 gastric juice, 2 pleural liquid and 1 sputum.

M. abscessus: 7 sputum, 2 bronchoalveolar lavage.

M. avium Complex: 2 fine needle aspiration biopsies (FNAB), 1 gastric juice and 1 bronchoalveolar lavage.

M. lentiflavum: 2 FNAB

Five out of 22 patients were cystic fibrosis patients, in 4 of them the microorganism isolated was M. abscessus. In the other one, M. lentiflavum was recovered.

Conclusions: The most common specimens received were those of the respiratory tract, where M. tuberculosis was the most prevalent mycobacteria followed by M. abscessus. On the other hand in FNAB specimens the species isolated were M. avium Complex, and M. lentiflavum. M. abscessus is the most common mycobacteria isolated in cystic fibrosis patients in this population during this period.

Session Details

Date: 10/04/2010
Time: 00:00-00:00
Session name: Abstracts 20th European Congress of Clinical Microbiology and Infectious Diseases
Location: Vienna, Austria, 10 - 13 April 2010
Presentation type:
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