Can the nucleic acid amplification test be an alternative to the serologic tests? Aprospective study results of 18,200 blood donors from Turkish Red Crescent

Abstract number: R2192

Kosan E., Kocazeybek B., Altunay H., Aymelek M., Alan E., Saribas S., Aslan M., Yenen O.S., Yuksel P., Birinci I., Kirali K., Aksoy A.

Aim: Serologic tests having high sensitivity and specificity are used in order to prevent contamination with infectious agents from blood and blood products for transfusion safety.. Present serologic tests have problems like having low sensitivity and weak detection capacity of infectious agents in "window period". We aimed to test the use of NAT (Nucleic Acid Amplification Test) in routine blood screening in Blood Banking.

Method: We used Procleix ultrio (Chiron, USA) test kit based TMA (Transcription Mediated Amplification) for NAT study in serum samples of 18200 donors who were applied to the Turkish Redcrescent between February 2007-September 2008. The NAT positive samples were studied twice. The discrimination of HIV, HCV and HBV of NAT positive samples were performed by Procleix Discrimination (Chiron, USA) test. Otherwise Micro ELISA were used parallely for routine serological screening of Anti-HIV, Anti-HCV and HBsAg with Vironoste HIV Uni-form, AG/Ab innotest HCV Ab and Hepanostica ultra HBsAg test kits.

Results: The results of serum samples with serology(+) and NAT(+) (13/18200 and 0.05%) for Anti-HIV, Anti-HCV and HBsAg were detected higher than other NAT studies and we also detected that a transfusion risk can be occurred in every 1400 transfusions.

Conclusion: We suggest that the reason for this higher positive NAT results can be due to donating status of blood donors. Blood donors(almost 100%) of our study were donating the first time. On the other hand, the cost of NAT test are very higher compared with other serologic tests. As a result of we suggest that it is not necessary to apply NAT tests to the all of the blood donors because of the NAT tests are not being cost-effective. We can also advice to apply the NAT tests to the blood donors who will donate the first time.

Table 1. Serologic tests and NAT results

PatternsAnti HIV/1–2Anti-HCVHBsAg
Serology(+) NAT(-)195917
Sero1ogy(-) NAT(+)-211
Serology(+) NAT(+)39297

Session Details

Date: 16/05/2009
Time: 00:00-00:00
Session name: 19th European Congress of Clinical Microbiology and Infectious Diseases
Location: Helsinki, Finland, 16 - 19 May 2009
Presentation type:
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