Prevalence of Chlamydia trachomatis, Neisseria gonorrhoeae, herpes simplex virus type1,2 and papilloma virus in pregnant women in central Greece
Abstract number: R2167
Klapsa D., Skentou H., Stavropoulou M., Damani A., Anastassiadou F., Messinis I., Petinaki E.
Objective: To examine the prevalence of genital pathogens including Chlamydia trachomatis, Neisseria gonorrhoeae, Herpes simplex virus types 1 and 2 (HSV1, HSV2) and Papilloma virus (HPV) among asymptomatic pregnant women of Central Greece.
Methods: A total of 269 pregnant women during the first trimester of pregnancy, aged from 18 to 42 years old, presented during 2008 at the University Hospital were included. Cervical swabs were obtained and DNA extraction was performed in all specimens. The presence of C. trachomatis was examined firstly by direct C. trachomatis antigen detection (Cypress Diagnostics) and by Real Time PCR (Nanogen Advanced Diagnostics). DNA of N. gonorrhoeae was detected by a home-made PCR, while DNA of HSV1 and HSV2 were detected by Real-Time PCR (Nanogen Advanced Diagnostics). The presence of HPV DNA and oncogenic potential was assessed using PCR followed by a restriction method (Maxim).
Results: Four out 269 women (4.8%) were positive by RT-PCR for C. trachomatis; the direct detection of C. trachomatis antigen was identified in only one out the four cases. In addition, two women (2.4%) were positive for HPV type 16. DNA of N. gonorrhoeae, of HSV1 and HSV2 were not found in any women.
Conclusions:C. trachomatis and HPV type 16 were identified in asymptomatic pregnant women in Central Greece. These results support a strategy of screening pregnant women for these pathogens (followed by treatment of infections), which could be integrated into routine pregnancy care in our area.
|Session name:||19th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Helsinki, Finland, 16 - 19 May 2009|
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