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Genotypes of Helicobacter pylori isolated from the patients with hepato-biliary diseases

Abstract number: R2156

Isaeva G.S., Abuzarova E.R., Valeeva J.V., Pozdeev O.K.

Objective: To evaluate the incidence of H. pylori virulence genes in the liver biopsies and the bile specimens.

Method: 50 patients with chronic cholecystitis and 50 patients with chronic alcoholic cirrhosis were included in our study. The presence of genes H. pylori was confirmed by polymerase chain reaction (PCR) using H. pylori specific primers for ureC, babA2, cagA, vacA ("Helicopol") according to the recommendations of the manufacturer "Lytech", Moscow (Russia).

Results:H. pylori DNA was isolated from the bile and liver biopsies samples in 54% cases of chronic noncalcular cholecystitis and 72% cases of cirrhosis. Results of H. pylori genotyping are presented in the table.

Conclusions: The prevalent genotype of H. pylori isolated from the patients with hepato-biliary disorders is UreC positive VacAs1 m2 positive CagA and BabA negative. The received data indicate the possible role of CagA negative H. pylori in biliary tract and liver diseases.

Table. Genotypes of H. pylori isolated from the bile and liver biopsies

H. pylori genesn (%)
 Chronic cholecystitis (N = 27)Cirrhosis (N = 36)
vacAs115 (55.56±9.56%)15 (41.67±8.22%)
vacAs23 (11.11±6.05%)7 (19.44±6.6%)
vacAs1/s22 (7.4±5.04%)0
vacAm104 (11.11±5.24%)
vacAm214 (51.85±9.66%)18 (50±8.33%)
vacA-7 (25.93±8.43%)14 (38.89±8.13%)
cagA+12 (44.44±9.56%)0
cagA-15 (55.56±9.56%)36 (100%)
babA2+8 (29.63±8.79%)0
babA2-19 (70.37±8.76%)36 (100%)

Session Details

Date: 16/05/2009
Time: 00:00-00:00
Session name: 19th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Helsinki, Finland, 16 - 19 May 2009
Presentation type:
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