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Identification of hepatitisB virus genotype in patients with hepatocellular carcinoma in Iran

Abstract number: R2153

Aghakhani A., Hamkar R., Mohraz M., Zamani N., Eslamifar A., Banifazl M., Adibi L., Ramezani A.

Objectives: Hepatitis B virus (HBV) is one of the major causative agents of hepatocellular carcinoma (HCC) in Iran. The development of HCC in patients with HBV infection has been associated with specific HBV genotypes. The aim of this study was to identify the HBV genotypes in patients with HCC in Iran.

Methods: This study was performed on paraffin-embedded tissue samples of 40 patients (31 males and 9 females) with HBV-associated HCC. HBV genotyping was performed by nested PCR-mediated amplification of the target sequence. PCR products were sequenced, and the genotype of each HBV sequence was determined by comparison with sequences of known genotypes in the GenBank and Phylogenetic tree was constructed.

Results: Phylogenetic analysis revealed that all of the HBV isolates were clustered in the genotype D.

Conclusion: Our results concurred with other reports from Iran all showing that genotype D is the only detectable genotype in different clinical forms of HBV infections in this country.

Session Details

Date: 16/05/2009
Time: 00:00-00:00
Session name: 19th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Helsinki, Finland, 16 - 19 May 2009
Presentation type:
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