Expression of interferon-induced microRNAs in patients with chronic hepatitisC virus treated with pegylated interferon alpha
Abstract number: P2022
Scagnolari C., Zingariello P., Selvaggi C., Zoccoli A., Vecchiet J., Cicetti S., Pizzigallo E., Antonelli G.
Objectives: In order to further elucidate the determinants of response to interferon (IFN) therapy in patients with chronic hepatitis C, a gene expression analysis of cellular microRNAs (miRNAs), which has been previously reported to be involved in IFN-mediated antiviral activity against hepatitis C virus (HCV), has been performed.
Methods: The expression of several miRNAs (mir-1, mir-30, mir-128, mir-196, mir-296) was retrospectively measured in peripheral blood of mononuclear cells (PBMC) derived from 12 patients with HCV before and after 12 hours from the first injection of IFN. Gene expression analysis of MxA, a well-characterised IFN type I-induced gene, was also performed as positive control.
Results: PBMC from patients with HCV express all examined miRNAs but their levels showed high variability (coefficient of variation >100%). In addition we observed that the overall expression of miRNAs was significantly different between patients with HCV and healthy subjects. When levels of the above miRNAs were measured 12 hours after the first injection of IFN, increases in expression levels of these miRNAs were observed in a percentage of patients ranging from 33.3% to 66.6% depending on the type of miRNA examined.
Conclusion: These findings suggest that miRNAs can be differentially induced by IFN treatment in HCV positive patients. Given the important role of miRNAs in defending the host against virus infection it is possible that such miRNAs may represent an important determinant of the clinical outcome of IFN therapy in HCV infection.
|Session name:||19th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Helsinki, Finland, 16 - 19 May 2009|
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