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Molecular analysis of a determinant region of the Sgene of HBV from healthy subjects with occult HBV infection in Istanbul

Abstract number: P2014

Midilli K., Aslan M., Ergin S., Yuksel P., Saribas S., Kosan E., Altunay H., Kucukbasmaci O., Kaygusuz A., Torun M., Caliskan R., Kocazeybek B.

Objective: HBV DNA and AntiHBc antibody positivity without HbsAg is defined as occult HBV infection, which has been detected at varying rates according to the local prevalence of HBV infection. Although in subjects with occult HBV infection "a" determinant region containing a dominant B cell epitope for anti-HBs response shows higher amino acid substitutions compared to HbsAg positive carriers, the exact mechanism of occult HBV infection is not fully elucidated. In this study we aimed to determine the mutations in the "a" region of S gene in subjects with occult HBV infection.

Methods: Eighty three blood donors positive for anti-HBc antibody alone were screened for HBV DNA by PCR targeting S gene. Following the sequencing of the PCR amplicons, mutations with a potantial of antigenic change in the "a" determinant region of the S gene were sought.

Results: Eight of 83 subjects (9.63%) were positive for HBV DNA. Based on the amino acid sequences between 101–180 all of them were ayw2 subtype and had no mutations in the "a" determinant region which might be related with antigenic change in the major B cell epitope.

Conclusion: Other mechanisms then the mutations in S gene of HBV may be involved in the occult HBV infections in our region with the exclusive predominance of the HBV genotype D.

Session Details

Date: 16/05/2009
Time: 00:00-00:00
Session name: 19th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Helsinki, Finland, 16 - 19 May 2009
Presentation type:
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