Clinical evaluation of a new commercial PCR-DNA microarray system for simultaneous detection of 17 respiratory viruses in French infants hospitalised for acute bronchiolitis
Abstract number: P1936
Andreoletti L., Huguenin A., Moutte L., Abely M., Bessaci K., Renois F., Nenninger T., Carrat F., Leveque N.
Background: Rapid testing for viral infection is recommended in infants who require admission to hospital with acute bronchiolitis in order to guide cohort arrangements or to improve therapeutic management in case of respiratory distress. Clinical virology laboratories used traditional methods as direct fluorescent-antibody assay (DFA) and culture for the detection of six or seven conventional respiratory viruses. In the present study, a new commercially available assay using RT-PCR followed by microarray detection assay designed for detection of 17 pathogenic respiratory RNA viruses was evaluated by testing clinical samples of infants hospitalised for bronchiolitis.
Methods: From October 2007 to December 2008 for we retrospectively selected 65 infants (mean age: 3.5 months, SD: 3.2 months) admitted to the paediatric department (University Hospital Center of Reims, France) for acute bronchiolitis. Infants with congenital heart disease or with a chronic genetic or acquired-immunodepression were excluded. Nasopharyngeal aspirate samples of the selected patients were tested by DFA and cell-culture detection assays and by microarray detection assay (Clart Pneumovir Version 3.0, Genomica, Madrid, Spain) for the presence of respiratory viruses.
Results: One or more potential causative viral agents were detected in 47, 51, 63 of 65 samples by viral culture, DFA and the microarray detection assay, respectively (P < 10-3). The frequency of detection of conventional respiratory viruses appeared to significantly higher using the microarray assay than using the classical techniques (P = 0.02). The Pneumovir assay detected 43 mixed infections that the most common associations were: adenovirus and RSV (26%), bocavirus and RSV (23%) and metapneumovirus and RSV (23%). Mixed infections appeared not to be statistically associated with the severity of the disease or secondary hospitalisation events for acute bronchiolitis or asthma within 6 months after the time of inclusion.
Conclusion: The use of this PCR-DNA microarray system in clinical virology practice allows a rapid and accurate detection of conventional and newly discovered viral respiratory pathogens in infants hospitalised for acute bronchiolitis. Moreover this new assay would be of major interest for the development of future therapeutic and preventive strategies to fight against the viral causes of bronchiolitis.
|Session name:||19th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Helsinki, Finland, 16 - 19 May 2009|
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