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Detection of antibodies to West Nile virus in people with schizophrenia using recombinant prM and Eproteins produced by COS1 cells

Abstract number: P1930

Aslan M., Kocazeybek B., Turan N., Yilmaz H., Karakose A.R., Yuksel P., Saribas S., Cakan H., Bunning M., Caliskan R., Torun M.

Aim: Antibodies to West Nile virus (WNV) have been found in many warm blooded animals and people and cause fever and encephalitis. As it is known, neurotropic viruses like WNV and Borna disease virus with a predilection for latency are potential associates. However, the role of WNV infection in schizophrenia is not well documented. Therefore, we aimed to investigate the frequency of antibodies to WNV and its association with neurological diseases like schizophrenia.

Method: 200 sera from patients with schizophrenia were collected from Bakirkoy Mental Health Hospital.100 sera from healty controls (HC) were collected from blood donors and 100 sera from the patients with other anxiety and depressive disorders were collected from the Psychiatry Department of the Cerrahpasa Medical Faculty. HC were blood donors with no history of non-progressive and non-infectious neurological disorders. The sera were analysed by ELISA using the recombinant antigens (prM and E) of WNV produced by COS-1 transformed cells. The home-made of ELISA showed the optimal concentration of antigen as 6 ug/mL and the optimal dilution of serum as 1/40. Human sera were therefore tested at this dilution. Each serum was tested in antigen coated, non-coated and COS-1 cell-coated wells to measure the background optical density. Sera that read at least 2-fold higher compared with background were considered positive

Results: We detected higher WNV antibody titers only in one case (no: 128). Borna virus IgG was also detected. No positivity was detected in any other patients and HC.

Patient (no:128): She is 70 years old and born in Adana city (Cukurova region of Turkey) which the endemic diseases transmitted with vectors (especially mosquito) are most commonly found. She was hospitalised in Bakirköy Mental Hospital for 40 years and still taking antipsychotic therapy. WNV antibody titer was highly elevated in this patient (Patient OD = 1.479, OD of control = 0.240).

Conclusion: In conclusion, the results of this preliminary study showed that antibodies to WNV in people do not seem to be frequently associated with schizophrenia and other psychatric disorders. In spite of that, detecting high WNV antibody titres in one patient suggests us to consider the possibility of relationship between WNV infections and psychiatric diseases in endemic areas. However, a secondary test is needed. Therefore, we have extracted RNA from the blood of those patients and the presence of WNV will be analysed by real-time RT-PCR.

Session Details

Date: 16/05/2009
Time: 00:00-00:00
Session name: 19th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Helsinki, Finland, 16 - 19 May 2009
Presentation type:
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