Staphylococcus aureus methicillin-resistant colonisation among healthcare workers at a general hospital

Abstract number: P1885

Álvarez-Fernández M., Potel-Alvarellos C., Constenla L., Prieto P., Álvarez-García P., Barbeito V., Alonso-Troncoso I.

Objectives: Studies of MRSA colonisation among health care workers (HCW) are important in order to control the emergence and spread of this organism in health-care institutions. The goal of the study was to estimate the prevalence of MRSA colonisation in HCW.

Methods: A total of 67 HCW were studied from January-February 2007, a nasal swab was obtained from each participant in the study. The nasal swabs were cultured on MRSA ID media(bioMérieux). The cultures were read at 24 and 48 hours. The MRSA phenotype was confirmed by the identification of the mecA by PCR. The genetic relatedness of the isolates was studied by RFLP (cfo I)-PCR of the coa gene. The spa, MLST, SCCmec, and agr types were studied. The presence of the pvl genes were studied by PCR.

Results: Six HCW were colonised by MRSA strains (8.9%). The strains according to the RFLP (cfo I)-PCR, were identified as two clones. The isolates belong to the t008-ST8-IV-agr 1 (CC8) (n = 2), and t018-ST36-II-agr 3 (CC30) (n = 4). Both clones were PVL negative.

Conclusion: The colonisation of HCWs with the epidemic community USA 300 related clone (t008-ST8-IV-agr1) is a matter of concern. The other clone belongs to the international British EMRSA-16 (t018-ST36-II-agr 3) that is endemic in the studied hospital. HCWs could be the reservoir for the spread MRSA strains.

Session Details

Date: 16/05/2009
Time: 00:00-00:00
Session name: 19th European Congress of Clinical Microbiology and Infectious Diseases
Location: Helsinki, Finland, 16 - 19 May 2009
Presentation type:
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