First blaVIM positive metallo-lactamase producing Enterobacter cloacae isolate in Germany

Abstract number: P1691

Schaumann R., Genzel G.H., Adler D., Stingu C.S., Rodloff A.C.

Objectives: The frequency of metallo-b-lactamase (MBL) producing strains of Pseudomonas spp. is increasing worldwide. Nowadays, also MBL producing Enterobacteriaceae are detected in clinical specimens creating significant therapeutic problems, since these strains are not only resistant to all b-lactams but often posses further resistance mechanism. Here we report about an Enterobacter cloacae strain with elevated MIC values against carbapenems isolated from clinical specimens at an University Hospital in Germany.

Methods: A Gram-negative rod isolated from multiple blood cultures of a haematological patient of the University Hospital of Leipzig, Germany was identified by the ID 32 E system. MICs against 23 antimicrobial agents were determined by broth microdilution. Furthermore, MBL test was carried out by Etest and PCRs were performed for blaVIM and blaIMP genes.

Results: The isolate was identified as E. cloacae. MICs for imipenem, meropenem and ertapenem were 4 mg/L, 4 mg/L, and 8 mg/L, respectively. MIC for imipenem determined by Etest for MBL detection was >32 mg/L and for imipenem plus EDTA 2 mg/L. Thus, the strain was identified as a MBL producer. The PCR was positive for the blaVIM but negative for the blaIMP gene.

Conclusion: To our knowledge we report here the first blaVIM positive MBL producing E. cloacae isolated from clinical specimens in Germany.

Session Details

Date: 16/05/2009
Time: 00:00-00:00
Session name: 19th European Congress of Clinical Microbiology and Infectious Diseases
Location: Helsinki, Finland, 16 - 19 May 2009
Presentation type:
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