Tigecycline and polymorphonuclear leukocyte function
Abstract number: P1653
Naess A., Andreeva H., Sørnes S.
Objectives: Tigecycline is an antibiotic which achieves high intracellular concentrations in polymorphonuclear leukocytes (PMNs). To evaluate the effects of tigecycline on the function of human PMNs, PMNs from fresh whole blood were incubated with tigecycline dilutions (0.1 to 100 mg/L).
Methods: Phagocytosis and oxidative burst induced by Staphylococcus aureus, as well as PMN Fcgamma- and complement receptors, were measured by flow cytometry.
Results: Tigecycline had no effect on the phagocytosis or oxidative burst induced by S. aureus. However, incubation with tigecycline was associated with small but statistically significant decreases in the density of PMN complement receptors CD11b and CD35 (all concentrations) and Fcgamma receptors CD16 (100 mg/L) and CD32 (10 and 100 mg/L), but not in the percentages of receptor-bearing cells, except for small reductions in the proportions of CD16 positive cells at 10 and 100 mg/L of tigecycline.
Conclusion: Tigecycline was thus associated with decreased density of human PMN complement and (at high concentrations) Fcgamma receptors. Although statistically significant, these differences were small and did not influence PMN function as measured by phagocytosis and oxidative burst induced by S. aureus.
|Session name:||19th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Helsinki, Finland, 16 - 19 May 2009|
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