Epidemiology and drug resistance pattern of cholera outbreak in summer 2008 in Iran

Abstract number: P1537

Rahbar M., Zahraei M., Omidvarnia A., Afshani M.T., Glami M., Gholami H., Islami P., Mohammad Zadeh M.

Objective: Cholera is an endemic disease in Iran. The aim of this study was to determine epidemiology and antimicrobial susceptibility patterns of Vibrio cholerae O1 biotype EL Tor in summer of 2008 in Iran

Methods: Stool samples were collected from patients suspected to have cholera admitted to hospitals and clinics. Specimens examined by conventional bacteriological methods. All isolates were sent to cholera reference laboratory for confirmation, stereotyping and susceptibility testing. Antimicrobial susceptibility testing was performed by disk diffusion methods as recommended by Clinical laboratory standard Institute (CLSI). Demographic data collected from questioner forms. The antimicrobial drugs testes included Ampicillin (AM), Co-trimoxazole (SXT), Ciprofloxacin (CI), Tetracycline (TC), Erythromycin (EM), Choleramphenicol (C), cefexime (CFM) Furazolidone (F). Nalidixic acid (NA). The E-test MIC method used for detection of minimal inhibitory concentration (MIC) for erythromycin.

Results: In total 220 patients diagnosed clinically and laboratory confirmation to have cholera Of 220 cases 199 serotypes were inaba and 21 cases were Ogawa. Disease reported from thirteen provinces. The majority of cases were from Tehran, Qum and Zahedan with 56, 26 and 25 cases respectively. 24 (11%) of patients were under 15 years old and 196 (89%) of patients were older than 15 years. 149 (68%) of patients were male and 71 (32%) were female. 129 (59%) of patients had Iranian nationality, 79 (36%) were from Afghanistan and 12 (5%) from Pakistan. All isolates were resistant to co-trimoxazole, nalidixic acid, furazolidone, and intermediate to chloramphenicol. All isolates were susceptible to tetracycline, ciprofloxacin, and erythromycin. MIC ranged1–1.5 mg/ml for erythromycin. The antimicrobial results and pulse field gel electrophoresis (PFGE) showed that all isolates had the same epidemiology and susceptibility patterns.

Conclusion: Our study reveals that in recent outbreak caused by V. cholerae EL Tor serotype Inaba were the predominant serotype. All isolates were resistant to co-trimoxazole, nalidix acid and furazolidon.

Session Details

Date: 16/05/2009
Time: 00:00-00:00
Session name: 19th European Congress of Clinical Microbiology and Infectious Diseases
Location: Helsinki, Finland, 16 - 19 May 2009
Presentation type:
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