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One-year perspective study on prevalence and characterisation of diarrhoeagenic Escherichia coli isolated from children, beef and cattle in Tehran, Iran

Abstract number: P1247

Jafari F., Tajbakhsh M., Torabi P., Azizmi-Rad M., Arabshahi M., Dabiri H., Nochi Z., Dezfolian A., Aslani M.M., Kashi L., Zali M.R.

Objective: Several routes exist for human infection with Diarrhoeagenic Escherichia coli (DEC) and Meat and it's products remains a main sources. The purpose of this study was to investigate the prevalence of five important categories of DEC in Cattle faeces, Beef and children with acute diarrhoea in Tehran, Iran.

Method: From November 2007 to 2008, four hundred and sixty six samples, including 222

Cattle faeces collected from healthy cattle (1–3, 4–6 and >6 months) in farm, 104 beef samples at the slaughterhouses and 140 stool specimens from children (Age categories: 0–6, 7–12, 13–24 and 25–60 months) with diarrhoea, who reffered to Tehran children's hospital were tested. All samples were investigated and identified for DEC and their virulence genes (stx1, stx2, eae, Lt, St, ial, ipaH, bfp, ttc, O157, H7, SOD and a-hly genes) by Conventional and Molecular techniques.

Result:A total of 270 (58%) of 466, one hundred and sixty seven (75.2%) of 222 faecal samples which, collected from healthy cattle in farm, 81 (77.8%) of 104 beef samples at the slaughterhouses and 13 (9.2%) of 140 stool specimens from children with diarrhoea were positive for the stx genes (STEC). Of the 270 STEC isolates, 15% harboured the stx1 gene, 23% harboured the stx2 gene and 62% carried both stx1 and stx2 genes. The eae gene, was significantly associated with the stx1 and stx2 genes in Meat samples (38%) and Cattle faeces (51%). 98 of 167 STEC positive, which were detected from animal faeces were carried eae gen (58.7%). Enteropathogenic E. coli (EPEC) strain was isolated from 23% of bovine faeces, 19% of beef samples, and 14.1% of stool specimens of children. A PCR indicated that 6.8%, 5%, 14%, 10.7% and 13.6% strains carried the a-hly, lt/st, ipaH, ial and Pcvd432 genes in stool specimens of children respectively. SOD gene was positive in 18.6%, 17.3% and 37.43% of STEC strains, wich are detected from Children, Meat and Cattle faeces respectively. Bloody diarrhoea was observed in 30.7% of children's stool.

Conclusion: The Result showed the most common DEC in children was Enteropathogenic E. coli (14.1%) and STEC was isolated from large number of the Meat samples (77.8%). Since the STEC are spread only via faecal excretion, at present it is most important to reduce the faecal shedding and to avoid faecal contamination of food of animal origin. In detail prophylactic hygienic measures concerning the Farm management, the Feeding hygiene, the Meat hygiene as well as Food hygiene are need.

Session Details

Date: 16/05/2009
Time: 00:00-00:00
Session name: 19th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Helsinki, Finland, 16 - 19 May 2009
Presentation type:
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