CTX-M-15 flanked by ISEcp1 and orf477 is the most frequent ESBL type in E.coli and K.pneumoniae from Bochum, Germany
Abstract number: P1198
Kaase M., Peters Y., Szabados F., Anders A., Sakinc T., Gatermann S.
Objectives: Therapeutic options in enterobacteriaceae are limited due to an increasing prevalence of extended-spectrum b-lactamases (ESBL). A worldwide increase of CTX-M b-lactamases has been described and the insertion element ISEcp1 plays a role in the dissemination of those b-lactamases. However little is known about the molecular epidemiology of ESBL in Germany.
Methods: From January 2006 to April 2007 E. coli (n = 102) and K. pneumoniae (n = 75) ESBL isolates were collected from two hospitals. ESBL types were determined by PCR and sequencing. PCRs for ISEcp1 and orf477 flanking CTX-M genes were performed as described before.
Results: 59.8% of all E. coli ESBL isolates carried CTX-M-15, 19.6% CTX-M-1, 8.8% CTX-M-14. In K. pneumoniae ESBL isolates CTX-M-15 was found in 49.3% and CTX-M-1 in 45.3%. The remaining isolates carried either CTX-M-3, CTX-M-9, SHV-12 or SHV-28.
ISEcp1 upstream of CTX-M was found in 91.8% and 35.1% of CTX-M-15 and CTX-M-1 genes, respectively. orf477 downstream of CTX-M was found in 98% and 59.2% of CTX-M-15 and CTX-M-1 genes, respectively.
Conclusion: ESBLs of the CTX-M-1 cluster, especially CTX-M-15, were the most frequent types in our area similar as in some but not all European countries. The high prevalence of ISEcp1 upstream of CTX-M emphasizes its role in the dissemination of this resistance gene.
|Session name:||19th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Helsinki, Finland, 16 - 19 May 2009|
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