Evaluation of a monoclonal-based antigen in stool enzyme immunoassay for diagnosis of Helicobacter pylori infection in Spanish children
Abstract number: P1163
Agudo S., Alarcón T., Urruzuno P., Somodevilla Á., López-Brea M.
Objectives:Helicobacter pylori infection can be diagnosed by invasive and no invasive methods. Invasive tests, including culture, histology and rapid urease, it is necessary to do a endoscopy to obtain biopsies of the gastric mucosa and non invasive techniques such as the urea breath test, serology or detection of H. pylori antigen in stool specimen (HpSA). In this study, we use a H. pylori stool antigen test as non-invasive diagnostic methods and we compared with diagnosis based on endoscopic biopsy-based methods (culture and urease test).
Methods: 50 samples of biopsies obtained from paediatric pacients with gastric symptoms before initiation of any therapy against H. pylori, received at the Department of Microbiology (Hospital Universitario de La Princesa, Madrid) from January 2006 to November 2008, were cultured according to standard microbiological procedures and all colonies suggestive of H. pylori were tested by Gram-strain, oxidase and urease tests to confirm the identificacion. Children also donated a sample of stool. Stool specimens from these patients were examined by rapid STRIP HpSA. (Pylori-Strip, Coris, Bioconcept), what are commercially available enzyme-linked immunosorbent assay based technology. The sensitivity and specificity were calculated for no invasive test used in this study.
Results: For these 50 children, 40 (80%) were diagnosed as positive and 10 (20%) were diagnosed negative for H. pylori infection by the gold standard methods (culture and urease). Whereas 37 (74.5%) were positive and 13 (26%) were diagnosed negative by the rapid STRIP HpSA test. The sensitivity and specificity were 92.5% and 100%, respectively.
Conclusion: Stool antigen test had high sensitive and specific for diagnostic of H. pylori. The non invasive test could be used as a routine diagnostic tool in the microbiology laboratory for assenssing clinical significance and eradication control of H. pylori, because it is more comfortable for the patients, especially for children and it is possible to obtain results rapidly without the need for sophisticated laboratory equipment.
|Session name:||19th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Helsinki, Finland, 16 - 19 May 2009|
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