Genotypic analysis of human Echinococcus granulosus strains in Turkey
Abstract number: P1058
Ergin S., Saribas S., Yuksel P., Zengin K., Midilli K., Adas G., Arikan S., Aslan M., Uysal H., Caliskan R., Oner A., Kucukbasmaci O., Kaygusuz A., Torun M., Kocazeybek B.
Objective:Echinococcus granulosus is the causative agent of hydatid cyst disease with considerable impacts on human/animal health and economy. Although cystic hydatid disease continues to be endemic in our country, molecular epidemiologic data regarding the genotypes of E. granulosus strains infecting human beings are limited. The aim of this study was the molecular characterisation of E. granulosus strains obtained from human.
Methods: Between March-December 2008 cyst contents of 21 patients were collected during the to surgery in the surgery departments of different hospitals in Istanbul. The samples were checked for the presence of protoscoleces microscopically and were preserved in 70% ethanol at -20°C. Genomic DNA was extracted using a commercial DNA extraction kit (Nucleospin tissue kit, Macherey Nagel, Germany). A 446 bp. long part of mitochondrial c oxidase subunit 1 (CO1) gene of Echinococcus granulosus was amplified with PCR. PCR products were purified and sequnced in both directions with an automated DNA sequencer (ABI®, 310).
Results: Phylogenetic analysis showed that 16 of 21 human cysts belonged to the G1 genotype (common sheep strain) of E. granulosus. The sheep strain (G1 genotype) of E. granulosus was to be the predominant genotype present in humans in the study. This is the first molecular analysis performed on exclusively human strains of E. granulosus in our country.
Conclusion: According to the results of our study in our country, the E. granulosus strains of both human and animal origins belong tho the same genotype.
|Session name:||19th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Helsinki, Finland, 16 - 19 May 2009|
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