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Caenorhabditis elegans-based analysis for the host-pathogen interaction of Salmonella enterica subsp. enterica serovars isolated from indigenous vegetables and poultry meat in Malaysia

Abstract number: P1012

Khoo C.H., Cheah Y.K., Sim J.H., Lee L.H., Awang Salleh N.Z., Mohd Sidik S., Radu S., Sukardi S.

Caenorhabditis elegans (C. elegans) have been widely used to study infections with promising results. Moreover, as its genome is surprisingly similar to that of humans (40% homologous), C. elegans becomes suitable as a simple host model.

Objectives: The increase of Salmonella enterica (S. enterica) occurrence in local indigenous vegetables and poultry meat can be potential health hazards. This study aimed to investigate the pathogenicity and persistent infection of various serovars S. enterica using C. elegans as a simple host model.

Methods: A total of S. enterica isolates (including 2 reference strains and 6 food sources) associated with 4 different serovars were tested. All S. enterica isolates were detected of virulence determinant by multiplex PCR. The virulence of S. enterica isolates in the C. elegans host model was evaluated by measuring the survival rate of worms fed on pure cultures of these isolates. Each assay was repeated 3 times for statistical analysis.

Results: Each S. enterica isolates under this study was found to possess up to 95% virulence genes. Result showed that different serovar have different mortality rate. The pathogenic S. enterica kills C. elegans faster than E. coli OPO50, which is the standard laboratory food strain. The time required for 50%C. elegans to die (TD50), which ranged from 3 to 4 days after ingesting various serovars of S. enterica compared to 17 days after ingesting the positive control strain E. coli OPO50. S. enterica shows similar persistency after 4 days of the infection which correlated to their TD50. Results from this study also revealed that the ability of S. enterica in killing of C. elegans correlates with its accumulation in intestine of the nematodes to achieve full pathogenicity.

Conclusion: The findings demonstrated that the virulence factors essential to mammalian pathogenesis also required for full pathogenicity in C. elegans.

Session Details

Date: 16/05/2009
Time: 00:00-00:00
Session name: 19th European Congress of Clinical Microbiology and Infectious Diseases
Subject:
Location: Helsinki, Finland, 16 - 19 May 2009
Presentation type:
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