Micro-organisms isolated from male urethral exudates during an 8year period in a Spanish teaching hospital

Abstract number: P690

Martínez M.C., Domingo D., Agudo S., Moreno M.J., Lopez-Brea M.

Aim: The main aim of this study was to describe the microorganisms isolated from male urethral exudates, from 2000 to 2008 at the Microbiology Department of La Princesa University Hospital in Madrid.

Methods: Eight hundred and eighty six samples obtained from urethral exudates from men with symptoms of urethritis were collected by standard procedures. They were examined by Gram stain and inoculated on blood agar medium, chocolate agar medium and modified Thayer-Martin medium and incubated at 37°C in O2, and 10% CO2 until 48 hours. Subsequently, they were identified by API NH (BioMerieux), by MicroScan (Dade Behring), CHROMagar Candida (Becton Dickinson/BBL) and Auxacolor.

Results: A total of 290 samples (32.73%) were considered of microbiological value and informed to the clinician. According to the positive samples, the percentage of isolated microorganisms were as follows:14.45% of Neisseria gonorrhoeae, 4.17% of Haemophilus parainfluenzae (groups II and III), 5.64% of Gram-positive cocci, 4.74% of Gram-negative rods and 3.72% of Candida sp.

The average age for each group of microorganism isolated was: 33.78 for N. gonorrhoeae, 32.65 for H. parainfluenzae, 49.18 for Gram-positive cocci, 67.88 for Gram-negative rods and 66.10 for Candida sp.

The resistance rate of N. gonorrhoeae to the antimicrobian agents studied, increased considerably during the period.

Conclusions: According to positive cultures, the most prevalent microorganism isolated from urethral exudates was Neisseriae gonorrhoeae, followed by Gram-positive cocci, Gram-negative rods, Haemophilus parainfluenzae and finally Candida sp. By age group, Neisseria gonorrhoeae and H. parainfluenzae were more prevalent in young adults, Gram-positive cocci had a scattered distribution and Gram-negative rods and Candida sp. were isolated more frequently in elderly. The high rates of resistance of N. gonorrhoeae may difficult the treatment of gonococcal infection in the next years.

Session Details

Date: 16/05/2009
Time: 00:00-00:00
Session name: 19th European Congress of Clinical Microbiology and Infectious Diseases
Location: Helsinki, Finland, 16 - 19 May 2009
Presentation type:
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