Symptomatic women with non-gonococcal, non-chlamydial cervicitis show a high incidence of Mycoplasma genitalium in Greece

Abstract number: P687

Karanikolas I., Baka S., Demeridou S., Kaparos G., Salomidou P., Genimata V., Arsenis G., Kouskouni E.

Objectives: The purpose of the present prospective study was to assess the incidence of Mycoplasma genitalium (M. genitalium) in a cohort of reproductive age women presenting with signs and symptoms of cervicitis since, to our knowledge, has not been previously reported in a Greek population.

Methods: Between March 2007 and March 2008 women of reproductive age presenting with signs and symptoms of cervicitis were invited to participate in the study. Eligible for our study were 150 women with nongonococcal, nonchlamydial cervicitis which previously presented at different hospitals and private practices with the same symptoms and did not receive therapy for mycoplasmas. In order to identify aerobic microorganisms cervical specimens collected from all study participants were inoculated on blood agar, MacConkey agar, Chapman and Sabouraud agar followed by incubation at 37°C for 24 hours, whereas anaerobic cultures were carried out on Wilkins-Chalgren agar at 37°C for 48 hours. The isolated strains were identified using the automated system VITEK 2 (BioMerieux, France). For the identification of Ureaplasma urealyticum and Mycoplasma hominis we used Mycoplasma IST 2 (BioMerieux, France). Samples were tested for M. genitalium using the hyplex® STD ID (BAG Health Care GmbH, Lich, Germany), a multiplex – PCR-ELISA system. Statistical analysis was performed using student t-test and chi-square test.

Results:M. genitalium was detected in 27 (18%) of the 150 women tested. Data regarding women tested positive for M. genitalium was compared to women who tested negative. The two groups did not differ in age (p = 0.85), in the number of children (p = 0.09) and in number of sexual partners (p = 0.64). Interestingly, a higher proportion of women not using condoms had M. genitalium isolated from their cervices (p = 0.021). Pruritus, in contrast to other symptoms, was more frequently associated with M. genitalium cervicitis (p = 0.05) while dyspareunia was significantly increased in the control group (p = 0.05) where other pathogens except M. genitalium where isolated. In 9 patients M. genitalium was the only pathogen isolated while in 12 cases was isolated together with Ureaplasma urealyticum. Finally, 6 patients presented with infection by all 3 mycoplasmas tested.

Conclusions: Symptomatic women harbour M. genitalium in their lower genital tract. Rapid detection by means of a multiplex – PCR-ELISA system is useful for a prompt and correct management of these women.

Session Details

Date: 16/05/2009
Time: 00:00-00:00
Session name: 19th European Congress of Clinical Microbiology and Infectious Diseases
Location: Helsinki, Finland, 16 - 19 May 2009
Presentation type:
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