In vitro activity of 13 antibiotics against CTX-M ESBL-positive and negative clinically significant Escherichia coli isolates from 8major hospitals in Kuwait
Abstract number: P663
Al Hashem G., Al Sweih N.A., Jamal W., Rotimi V.O.
Objective: The objective was to evaluate the antibiotic susceptibility of Escherichia coli, an important community and hospital pathogen, isolated from 8 Kuwait hospitals.
Methods: A total of 846 consecutive clinically significant strains of E. coli were studied during a one-year period. In vitro activity of 13 antibiotics against the isolates was determined by Etest. ESBL-production was assessed by ESBL-Etest method and confirmed by PCR technique. PCR amplicons positive for blaCTX-M were sequenced.
Results: About 69% of the E. coli isolates were highly non-susceptible to ampicillin with MIC90 of 256 mg/ml. Resistance to the 3rd generation cephalosporins ranged from 7.5% in Maternity hospital (MH) to 29% in Ibn Sina hospital (ISH); ciprofloxacin resistance rates ranged from 14% and 40%, respectively. Carbapenems and amikacin demonstrated excellent activities. Prevalence of ESBL-producing E. coli varied from hospital to hospital, with highest rate (32%) from ISH and lowest (4%) from Mubarak hospital and MH. MIC90 of cefotaxime, ceftazidime, cefepime and ciprofloxacin were >256, 64, >256 and >32 mg/ml, respectively for CTX-M-positive isolates versus 0.5, 1, 025 and 0.125 mg/ml for CTX-M-negative strains. Frequencies of CTX-M-positive isolates in cefotaxime MIC range of 12, 38, 916 and >16 mg/ml were 0, 4, 15 and 81%, respectively.
Conclusion: The prevalence of E. coli resistant to the 3rd generation cephalosporins and ciprofloxacin is at an unacceptable level. This is compounded by a high incidence of CTX-M ESBL-producing strains in almost all hospitals in Kuwait.
|Session name:||19th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Helsinki, Finland, 16 - 19 May 2009|
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