Fulminant deaths caused by Crimean-Congo haemorrhagic fever in Iran
Abstract number: P560
Chinikar S., Ghalyanchi Langeroudi A., Mirahmadi R., Ghaiasi S.M., Goya M.M., Shirzadi M.R., Moradi M., Afzali N., Jalali T., Zeinali M., Bouloy M.
Objectives: Crimean-Congo Haemorrhagic Fever (CCHF) disease is one of the most important infectious diseases in Iran. This arboviral disease is common between humans and livestock with a mortality rate around 50% whose agent is a virus of the genus Nairovirus and family Bunyaviridae which is transmitted from infected livestock to humans by the bite of Hyalomma ticks or directly between humans. CCHF has been reported from Africa, Eastern Europe, Asia and the Middle East. In addition to clinical signs, the diagnosis of the disease is based on finding anti-CCHF antibodies (IgM and IgG) and a fragment of the viral genome by molecular analysis (RT-PCR) in the patient's sera. In 1999, some cases of clinical findings have been reported from some provinces of Iran, especially the Chahar Mahal Bakhtiari and according to the studies of the laboratory of Arboviruses and Viral Haemorrhagic Fevers of the Pasteur Institute of Iran (National Reference Lab), the disease is endemic in Iran.
Methods: From 1999 to 2007, 207 sera of human dead cases suspected for CCHF have been sent for diagnosis to the Arbovirus Lab from all around the country, all have been analyzed for the presence of specific antibodies (IgM) against CCHF by ELISA and of the virus genome by RT-PCR.
Results: 64 sera were studied in 207 human dead cases infected by CCHF. In those cases with a molecular diagnostic positive response, in 67.2% of the cases no antibody against CCHF was observed. On the other hand in 9.4% only antibody response without viral genome presence has been observed. In 23.4% of the patients, both criteria of antibody response and molecular diagnosis have been found positive.
Discussion: The results of this study indicate that in a high proportion of fulminant deaths due to CCHF no immunological response has been observed and this can corroborate the virulence of the disease and the resulting mortality rate.
In this regard, for a better analysis of the pathogenicity of the disease, the determination of mediators in the serum (Such as Interleukins ...) and also the genetical study of intervening factors in the pathogenicity are underway in the different research projects of the laboratory.
|Session name:||19th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Helsinki, Finland, 16 - 19 May 2009|
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