Genetic diversity of human rabies virus isolated during 20002008 in Morocco
Abstract number: P558
Faouzi A., Anga L., Amarouch H., Nourlil J.
Objective: Rabies is an acute, progressive and fatal disease caused by an RNA virus of the Lyssavirus genus of the Rhabdoviridae family. The World Health Organization (WHO) estimates that rabies is related to the death of approximately 55,000 people per year. In Morocco human mortality due to rabies is estimated to be 20 deaths yearly. The aim of the present study was to determine the genetic diversity using sequence analysis of rabies strains isolated from humans in the Pasteur Institute of Morocco during 9 years.
Methods: Since 2000, 70 post-mortem brain samples from suspected rabid humans were collected from different areas of Morocco. Rabies virus was isolated from all samples and RT-PCR was used to amplify a 1300 nucleotide segment of the Nucleoprotein gene. Segment sequencing data was used for phylogenetic analysis.
Results: Comparisons of Nucleotide homology and phylogenetic tree analysis based on this sequence indicated that all the rabies virus isolates from Morocco belonged to genotype 1. Our result demonstrated a low diversity between different strains with high nucleotide homologies (99.1100%).
Conclusions: Data presented in this study demonstrate the importance of molecular protocol for diagnosis suspected rabid humans and to evaluate the genetic diversity of strains circulating in our country. This study showed that rabies virus isolates from Morocco have a close genetic relationship.
|Session name:||19th European Congress of Clinical Microbiology and Infectious Diseases|
|Location:||Helsinki, Finland, 16 - 19 May 2009|
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