Molecular characterisation of Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis clinical isolates in a tertiary-care hospital

Abstract number: P520

de Toro M., Torres M.J., Ruiz M., Aznar J.

Objective: To describe the prevalence of the recently renamed species Candida orthopsilosis and Candida metapsilosis in candidaemia and identified them in isolates recovered from different body sites.

Methods: Fifty-six clinical isolates recovered from blood cultures obtained from patients with candidaemia (study period 2003–2008), and 74 isolates from a variety of sources from some in-hospital and outpatient were studied. These isolates were previously identified as Candida parapsilosis by conventional laboratory tests. Molecular characterisation of the clinical isolates was performed by RAPD test using the primer RPO2 (5'-GCGATCCCCA-3'), and by BanI digestion profile analysis of a fragment of the secondary alcohol dehydrogenase gene. C. parapsilosis ATCC 22019, C. orthopsilosis ATCC 96139 and C. metapsilosis ATCC 96144 were also included as reference strains.

Results: Between 2003 and 2008, a total of 381 cases of candidaemia were detected in our hospital, and 95 (24.9%) of them corresponded to C. parapsilosis. Based on molecular criteria, 10 of the 130 studied isolates were identified as C. orthopsilosis (6 of them were bloodstream strains and 4 of them were from other sources), and 1 as C. metapsilosis (recovered from a skin sample). The remaining 119 isolates were identified as C. parapsilosis. Based on these results, C. orthopsilosis accounted for 7.7% (10/130) of all strains studied, but accounted for 10.7% (6/56) of the C. parapsilosis bloodstream strains and for 5.4% (4/74) of the strains from other sources. C. metapsilosis accounted for 0.77% (1/130).

Conclusions: The prevalence of the recently described specie C. orthopsilosis in candidaemia is significant and its characterisation can be recommended. Further studies are needed to establish the clinical significance of C. metapsilosis isolates in our area.

Session Details

Date: 16/05/2009
Time: 00:00-00:00
Session name: 19th European Congress of Clinical Microbiology and Infectious Diseases
Location: Helsinki, Finland, 16 - 19 May 2009
Presentation type:
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